2013 Fiscal Year Final Research Report
Development of proteomics technology using next generation sequencer
| Project/Area Number |
23651190
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Genome biology
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| Research Institution | The University of Tokyo |
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Principal Investigator |
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| Project Period (FY) |
2011 – 2012
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| Keywords | プロテオーム / 次世代シーケンサ / インタラクトーム / IVV |
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| Research Abstract |
In vitro virus (IVV) technology, a variation of mRNA display, is suitable for extensive protein-protein interaction analysis. Previous IVV method requires bait protein preparation which become the rate-limiting step. This work aimed to develop IVV method which is not need bait molecule purification. Chimeric DNA whose ends are corresponding to interacting pair can prepare by cross-linking of IVV molecule complex and following emulsion PCR, and next generation sequencing of the DNA can detect interacting pair data without bait preparation step. This novel method serves as the foundation of simple and comprehensive protein-protein interaction analysis.
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