2011 Fiscal Year Final Research Report

Exaustive screening of aberrant transcripts made by mature mRNA re-splicing in cancer cells

Research Project

Project/Area Number	23651198
Research Category	Grant-in-Aid for Challenging Exploratory Research
Allocation Type	Multi-year Fund
Research Field	Medical genome science
Research Institution	Fujita Health University
Principal Investigator	KAMEYAMA Toshiki 藤田保健衛生大学, 総合医科学研究所, 助教 (60298544)
Project Period (FY)	2011
Keywords	異常スプライシング / 成熟mRNA再スプライシング / 投縄状RNA中間体 / TSG101遺伝子 / FHIT遺伝子 / RNase R / RNA抽出法
Research Abstract	The length of the intron is widely diverse from under 100nucleotides to hundreds of thousands of nucleotides. Therefore, the lariat RNA intermediates, a by-product of splicing reaction, have various sizes. To sequence lariat RNA intermediates from cancer cells exhaustively using next generation sequencer, construction of high quality library from various size lariat RNA is essential. However, purification of high quality lariat RNA is very difficult because large lariat RNAs break easily biochemically and physically. We tested some RNA purification methods, and as a result, we find that handling very mildly during RNA purification like genomic DNA purification is very important for high quality lariat RNA purification. Now, we are trying to construct high quality lariat RNA library paying attention to this point.

(5 results)