2012 Fiscal Year Final Research Report
Inhibition of tumor suppressor protein p53-dependent transcription by a tetramerization domain peptide via hetero-oligomerization
| Project/Area Number |
23651210
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Living organism molecular science
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| Research Institution | Hokkaido University |
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Principal Investigator |
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| Project Period (FY) |
2011 – 2012
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| Keywords | 蛋白質 / 核酸 / 酵素 / 生体分子 / 遺伝子 |
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| Research Abstract |
In this study, we have developed a method for transient inhibition of p53-dependent transcription using a p53 tetramerization domain peptide that contains cell penetrating and nuclear localization signals. The peptide was efficiently introduced into cells and inhibited p21 expression via heterotetramerization with endogenous p53 protein. This method can be applied towards safe and efficient iPS cell generation.
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