2013 Fiscal Year Final Research Report
Development of the novel chemoselective capture method for sphingolipids
| Project/Area Number |
23651211
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Living organism molecular science
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| Research Institution | Hokkaido University |
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Principal Investigator |
MONDE KENJI 北海道大学, 先端生命科学研究科(研究院), 教授 (40210207)
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| Project Period (FY) |
2011 – 2013
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| Keywords | スフィンゴシン / グルタルアルデヒド / 捕捉 / 2-アミノ-1, 3-ジオール / 官能基特異的 |
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| Research Abstract |
To create new methodology of chemoselective catch toward sphingosine, large scale organic synthesis of target molecule was performed. Four standard samples having four different stereochemistries were prepared from D- and L-serine. The glutaraldehyde group was selected as functional group to react chemoselectively toward an 2-amino-1,3-diol function. The phenyl glutaraldehyde derivative was synthesized and connected with surface of the resin. The immobilization efficiency was confirmed by IR. The OPA derivatization method for detection of sphingosines reveals clearly its efficiency of the catching. To detect small amount of sphingosine in serum, downsizing of this new methodology was successfully performed utilizing the previously synthesize standard samples.
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[Journal Article] Salvileucalin C, a novel rearranged neoclerodane diterpene from Salvia leucantha2011
Author(s)
Aoyagi Y., Yamazaki A., Kato R., Tobe F., Fukaya H., Nishikawa T., Nakahashi A., Miura N., Monde K., Takeya K.
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Journal Title
Tetrahedron Lett.
Volume: 52
Pages: 1851-1853
Peer Reviewed
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