2012 Fiscal Year Final Research Report
Development and application of "artificial biotin" derivatives using methodologies of chemical biology
| Project/Area Number |
23651231
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Chemical biology
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| Research Institution | The University of Tokyo |
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Principal Investigator |
TERAI Takuya 東京大学, 大学院・薬学系研究科, 助教 (00508145)
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| Project Period (FY) |
2011 – 2012
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| Keywords | 化合物ライブラリー / 生体分子の化学修飾 |
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| Research Abstract |
In order to further sophisticate biotin-streptavidin system that is used as a universal binding pair in chemical biology, we screened a large library of approximately 160,000 compounds for novel ligands to streptavidin. As a result, we succeeded in obtaining new ligands that have affinities to the protein in the order of 1 μM. Moreover, we analyzed their binding mechanisms in detail by X-ray crystallography and computer simulation.
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