2012 Fiscal Year Final Research Report
Development of an activity quantitation method for signal transduction factors in living cells using inhibitors as probes
| Project/Area Number |
23651233
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Chemical biology
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| Research Institution | The University of Tokyo |
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Principal Investigator |
MAEDA Tatsuya 東京大学, 分子細胞生物学研究所, 准教授 (90280627)
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| Project Period (FY) |
2011 – 2012
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| Keywords | 活性依存型阻害剤 / mTOR / カルパイン / ワートマニン / TPCK / E-64-d / 抗ハ プテン抗体 |
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| Research Abstract |
Some inhibitors for signal transduction factors covalently attach to the target catalytic residues in their targets only when the targets are activated and the reactivity of the catalytic residues become high. A methodology was developed that exploits this reactivity as an activity quantitation method for signal transduction factors. To detect covalent modifications by inhibitors, western blotting using antibodies raised against the modification groups as haptens was used. To evaluate the methodology, three inhibitors targeting the TOR pathway or the yeast Rim101 pathway were tested.
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[Journal Article] Characterization of histidine-aspartate kinase HK1 and identification of histidine phosphotransfer proteins as potential partners in a Populus multistep phosphorelay2013
Author(s)
Hericourt F, Chefdor F, Bertheau L, Tanigawa M, Maeda T, Guirimand G, Courdavault V, Larcher M, Depierreux C, Benedetti H, Morabito D, Brignolas F, Carpin S
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