2012 Fiscal Year Final Research Report

Development of Fluorescent Probe for Visualization of Intracellular Chloride Anion

Research Project

Project/Area Number	23651238
Research Category	Grant-in-Aid for Challenging Exploratory Research
Allocation Type	Multi-year Fund
Research Field	Chemical biology
Research Institution	Kyushu University
Principal Investigator	OJIDA Akio 九州大学, 薬学研究院, 教授 (10343328)
Project Period (FY)	2011 – 2012
Keywords	クロライドイオン / 蛍光プローブ / バイオイメージング / 細胞機能
Research Abstract	We have developed a new acridinium amide-type fluorescent sensor for chloride anion. This probe showed a large fluorescent-off signal in response to chloride anion based on the collisional quenching mechanism in the biological relevantconcentration range (from 1 mM to 100 mM). Taking advantage of the sensing ability, we successfully applied this probe to the fluorescent bioimaging of intracellular chloride anion in living HEK293 cells. We have also developed another type of acridinium amide probe that can penetrate cell membrane, which we expect would be a valuable molecular tool for analysis of biological roles of chloride anion.