2013 Fiscal Year Final Research Report
Development of novel affinity purification method using metal enolate
| Project/Area Number |
23651244
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Chemical biology
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| Research Institution | The Institute of Physical and Chemical Research |
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Principal Investigator |
DODO Kosuke 独立行政法人理化学研究所, 袖岡有機合成化学研究室, 研究員 (20415243)
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| Co-Investigator(Kenkyū-buntansha) |
EGAMI Hiromichi 独立行政法人理化学研究所, 袖岡有機合成化学研究室, 客員研究員 (50553848)
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| Project Period (FY) |
2011 – 2013
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| Keywords | 有機化学 / 有機金属化学 / 金属エノラート / 固相反応 / タンパク質 / アフィニティー精製 |
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| Research Abstract |
To identify the binding proteins and binding sites of bioactive compounds, we planned ot develop novel affinity purification method using "metal enolate", which is derived from carbonyl compound and metal complex. As s result, by using solid-supported palladium complex, we successfully captured amino acid derivatives having carbonyl group as a palladium enolate in aqueous media. Moreover, we also succeeded in the reaction between electrophile having a fluorescent group and palladium enolate leading to fluorescent labeling.
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