2012 Fiscal Year Final Research Report
Development of activation technology for cell-free system under microwave irradiation
| Project/Area Number |
23655146
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Environmental chemistry
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| Research Institution | Tokyo University of Science |
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Principal Investigator |
YOSHIMURA Takeo 東京理科大学, 理工学部・応用生物科学科, 助教 (10580938)
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| Project Period (FY) |
2011 – 2012
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| Keywords | グリーンケミストリー |
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| Research Abstract |
New technology of protein expression is necessary for protein crystal structure, biomaterial and medical science. The cell free protein expression system is expected to lead the future bioengineering. To achieve acceleration of activity of enzymatic reactions, we prepared an original microwave applicator for microliter reaction scale. In this study, expression of GFP protein and DNA polymerization were demonstrated by using resonant cavity type of microwave applicator. As a result, we confirmed the microwave effectto enzymatic reactions.
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