2013 Fiscal Year Final Research Report

Preparation of environment adaptable micro-reactor based on erythrocyte ghost membrane; investigation by chemical luminescence

Research Project

Project/Area Number	23655148
Research Category	Grant-in-Aid for Challenging Exploratory Research
Allocation Type	Multi-year Fund
Research Field	Environmental chemistry
Research Institution	Hachinohe National College of Technology
Principal Investigator	OKUBO Satoshi 八戸工業高等専門学校, その他部局等, 教授 (90132555)
Co-Investigator(Kenkyū-buntansha)	SAITO Takayuki (10290686)
Project Period (FY)	2011 – 2013
Keywords	赤血球ゴースト膜 / リポソーム / ミクロ反応器 / 蛍光共鳴エネルギー移動
Research Abstract	In order to propose an environment adaptable micro-reactor, this research is the fundamental research to investigate the relation of luminescent reaction to fusion of erythrocyte ghosts from sheep blood. It was observed the extent of fluorescence resonance energy transfer(FRET) between two enclosed and fused fluorescent substances within each erythrocyte ghost with several micrometer size, and evaluated the fusion of the erythrocyte ghosts. As the results, it was shown that the FRET in the combined vesicles was observed in dependence on selection of temperature condition and the fusion methods, and it was revealed that the fused erythrocyte ghosts can be applied to a new type of micro-reactor.