2011 Fiscal Year Final Research Report
Development of T7 promoter controlled by DNA triplex formation
| Project/Area Number |
23655154
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Chemistry related to living body
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| Research Institution | Tokyo Institute of Technology |
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Principal Investigator |
SEIO Kohji 東京工業大学, 生命理工学研究科, 准教授 (20313356)
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| Project Period (FY) |
2011
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| Keywords | 三重鎖核酸 / 人工核酸 / T7-RNAポリメラーゼ / 転写制御 / 人工遺伝子 |
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| Research Abstract |
We introduce a pyrimidopyrimidoindole nucleobase(PPI) and pseudouridine as the analogs of cytosine and thymidine, respectively, in T7 promoter sequence. The artificial DNA was subjected to the transcription by usint T7-RNA polymerase. As the results, it was proved that the modified T7 promoter possessed the ability to promote transcription by T7-RNA polymerase. Moreover, it was also proved that the transctiption was inhibited by the addition of triplex forming oligonucleotide capable of recognizing the modified promoter sequence
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