2013 Fiscal Year Final Research Report

Screening of fluorescently detectable RNA using evolutionary molecular engineering

Research Project

Project/Area Number	23655165
Research Category	Grant-in-Aid for Challenging Exploratory Research
Allocation Type	Multi-year Fund
Research Field	Chemistry related to living body
Research Institution	National Institute of Advanced Industrial Science and Technology
Principal Investigator	KATO Yoshio 独立行政法人産業技術総合研究所, バイオメディカル研究部門, 主任研究員 (20415657)
Project Period (FY)	2011 – 2013
Keywords	核酸 / 蛍光性ＲＮＡ / 進化分子工学
Research Abstract	To obtain the spontaneously fluorescent RNA in cells, we have expressed the randomized RNA library in E. Coli and analyzed the fluorescent characteristics in E. Coli. We have constructed RNA library binding to FMN (flavin mono nucleotide), which emits fluorescence and ubiquitously dispersed all over cells, and expressed the cDNA library in E. Coli. We analyzed the fluorescent characteristics of 10000000 E.Coli clones, however, we have not obtained the expected fluorescent RNA yet.