2012 Fiscal Year Final Research Report

Development of method of inducing cell response by gene expression plasma flow

Research Project

Project/Area Number	23656128
Research Category	Grant-in-Aid for Challenging Exploratory Research
Allocation Type	Multi-year Fund
Research Field	Fluid engineering
Research Institution	Tohoku University
Principal Investigator	SATO Takehiko 東北大学, 流体科学研究所, 教授 (10302225)
Co-Investigator(Renkei-kenkyūsha)	JOHKURA Kohei 信州大学, 医学部, 准教授 (30303473)
Project Period (FY)	2011 – 2012
Keywords	バイオ流体力学 / プラズマ細胞応答誘導
Research Abstract	To transport of plasma stimuli to cell membrane, we found following results: 1. a primary streamer under water is much suitable to apply charged particles due to its low discharge current. 2. Hydrogen peroxide in the plasma-treated culture medium is not the inactivation factor of cell viability among chemical species when the plasma stimulus is weak though H2O2 is the main in activation factor in the strong plasma stimuli case.3. Analysis using AFM revealed morphologically the cell membrane surface and the plasma effect on the surface shape.