2013 Fiscal Year Final Research Report
Degradation of low concentration contaminants by oligotrophic bacteria
| Project/Area Number |
23656333
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Civil and environmental engineering
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| Research Institution | Tohoku Gakuin University |
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Principal Investigator |
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| Project Period (FY) |
2011 – 2013
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| Keywords | 貧栄養細菌 / フェノールヒドロキシラーゼ / トリクロロエチレン |
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| Research Abstract |
Oligotrophic phenol-utilizing bacterium, Cupriavidus sp. TW2, is able to degrade Trichloroethene (TCE) by phenol hydroxylase (PH) induced by phenol. Homologous recombination was carried out to insert the tac promoter (Ptac) upstream of PH genes for constitutive expression. The resultant strain, TW2-P, showed the higher TCE degradability without the phenol induction.
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