2012 Fiscal Year Final Research Report
Construction of versatile fluoroimmunoassay probe based on quench-release principle
| Project/Area Number |
23656517
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Biofunction/Bioprocess
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| Research Institution | Tokyo Institute of Technology (2012)
The University of Tokyo (2011) |
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Principal Investigator |
UEDA Hiroshi 東京工業大学, 資源化学研究所, 教授 (60232758)
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| Co-Investigator(Kenkyū-buntansha) |
OHASHI Hiroyuki 東京大学, 特任研究員 (40589454)
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| Co-Investigator(Renkei-kenkyūsha) |
OHMURO Yuki (MATSUYAMA Yuki) 東京大学, 特任研究員 (30571088)
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| Project Period (FY) |
2011 – 2012
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| Keywords | バイオセンサー |
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| Research Abstract |
With the aim of extending Quenchbody principle that can detect various antigens in a rapid and sensitive manner, an antibody binding protein probe that is fluorescence labeled at its terminus was constructed. When this probe was mixed with an anti-vimentin human Fab fragment, the dye of the probe was quenched by the Trp residues in the antibody. Moreover, when the antigen vimentin was added, dose-dependent release of the quenching was observed, thus resulted in successful detection of antigen by fluorescence increase.
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