2012 Fiscal Year Final Research Report
Turn-on split fluorescent tag responding to enzymatic ligation
| Project/Area Number |
23656518
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Biofunction/Bioprocess
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| Research Institution | The University of Tokyo |
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Principal Investigator |
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| Project Period (FY) |
2011 – 2012
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| Keywords | 蛍光標識 / 酵素 / 動物細胞 / タンパク質 / ペプチド / スプリットタグ / 細胞内導入 / ターンオンプローブ |
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| Research Abstract |
The labeling method consisting of a peptide tag and a fluorescent dye which can specifically label a target protein and turn on the fluorescence after labeling was developed. We found the pair of tag sequences and a dye which fluorescent intensities were increased 2.6 times after specific labeling via soltase-mediated ligation. Furthermore, to enable intracellular labeling, a method for intracellular specific ligation was successfully developed by transducing Streptococcus pyogenes sortase A into living mammalian cells.
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