2012 Fiscal Year Final Research Report
Challenge to microbial synthesis of block copolyesters by regulated gene expression
| Project/Area Number |
23656521
|
|---|
| Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Biofunction/Bioprocess
|
|---|
| Research Institution | Tokyo Institute of Technology |
|---|
Principal Investigator |
FUKUI Toshiaki 東京工業大学, 大学院・生命理工学研究科, 准教授 (80271542)
|
|---|
| Project Period (FY) |
2011 – 2012
|
| Keywords | 生物機能工学 / 生分解性プラスチック / 合成生物学 |
|---|
| Research Abstract |
This study aimed at microbial synthesis of polyhydroxyalkanoate (PHA) copolymers in which two kinds of monomers were highly block-covalently bonded by regulated expression of the biosynthetic genes. Firstly, copolymer biosynthesis pathways suitable for regulatory gene expression were established in Escherichia coli. Then, recombinant strains of E. coli equipped with a regulatory PHA biosynthesis pathways were constructed, and PHA biosynthesis by the strains and structural analyses of accumulated PHAs are nowunder progress.
|
