2012 Fiscal Year Final Research Report
Production of influenza vaccine using genetically manipulated chickens
| Project/Area Number |
23656523
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Biofunction/Bioprocess
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| Research Institution | Nagoya University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
ONO Etsuro 九州大学, 大学院・医学研究院, 教授 (00160903)
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| Project Period (FY) |
2011 – 2012
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| Keywords | 生物機能工学 / 感染症 / 糖鎖 / トランスジェニックニワトリ / ワクチン |
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| Research Abstract |
Vaccine against influenza virus is currently produced in developing chicken eggs. Allantoic membrane, the site for virus propagation for vaccine, does not express α2,6-linked sialic acid on cell surface, resulting poor infection/growth of human influenza virus. Therefore, chicken eggs with α2,6-linked sialic acid on allantoic membrane might be useful tool for vaccine production. To genetically modify chickens to express α2,6-linked sialic acid, the sialyltransferase gene was cloned and its enzymatic activity was confirmed. We also confirmed that ubiquitous actin promoter effectively expressed eGFP and single-chianed antibody in allantoic membrane. The sialyltransferase gene was inserted into the downstream of actin promoter of retrovirus vector construct. The packaging cell lines that produce high titer of retrovirus vector were successfully established. These are helpful to make transgenic chickens that express modified glycans on allantoic membrane.
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