2011 Fiscal Year Final Research Report
Effects of intestinal bacterial adhesion to fibers on intestinal microflora
| Project/Area Number |
23656534
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Biofunction/Bioprocess
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| Research Institution | Kansai University |
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Principal Investigator |
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| Project Period (FY) |
2011
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| Keywords | 生物機能工学 / 腸内細菌 / DnaK / セルロース / ムチン / 接着タンパク質 |
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| Research Abstract |
Surface proteins from Lactococcus lactis IL1403 cells purified by cellulose column were identified to be DnaK, GroEL, GAPDH and so on. The adsorption constants of the fluorescence labeled DnaK at pH 7.0 were estimated to be(2.7±0.9)×10^6、(2.5±0.5)×10^5、(3.7±2.2)×10^5、(8.7±3.2)×10^5 M^<-1> for cellulose, mucin, chitin and IL1403 cells, respectively. At pH 4.0, the adsorption constants for cellulose and mucin were estimated to be(5.7+-0.2) 10^6 and(4.4+-1.0) 10^6M^(-1), respectively. These results mean that lactic acid bacteria adhere to intestinal surface more tightly when they produce lactic acid.
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