2012 Fiscal Year Final Research Report
Affinity Maturation of Antibodies by Induction of Gene Conversion or Somatic Hypermutation.
| Project/Area Number |
23657002
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Genetics/Genome dynamics
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| Research Institution | The University of Tokyo |
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Principal Investigator |
OOTA Kunihiro 東京大学, 大学院・総合文化研究科, 教授 (90211789)
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| Project Period (FY) |
2011 – 2012
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| Keywords | 抗体遺伝子 / 突然変異 / 組換え / 抗体工学 / B 細胞 / 親和性成熟 |
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| Research Abstract |
In adaptive immune system, somatic hyper mutation in hyper-variable regions of antibody loci leads to affinity maturation of antibodies that strengthens the antigen binding activities. We previously developed an in vitro-based antibody design system using chicken B cell line DT40. This system, referred to as “ADLib (Autonomously Diversifying Library) system”, allows very rapid monoclonal antibodies production (within a week). In this study, ADLib system is newly equipped with the somatic hypermutation process, which is regulated by AID (auxin-inducible degron). In other words, affinity maturation is reconstituted in vitro by the use of DT40. Using this system, we can improve affinity of antibodies substantially.
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