Genomic target site recognition by SOX-partner factor complexes that underlies switching mechanisms in cell differentiation

2014 Fiscal Year Final Research Report

• Project/Area Number: 23657007
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Genetics/Genome dynamics
• Research Institution: Kyoto Sangyo University (2014); Osaka University (2011-2013)
• Principal Investigator: KONDOH Hisato 京都産業大学, 総合生命科学部, 教授 (70127083)
• Co-Investigator(Kenkyū-buntansha): KAMACHI Yusuke 大阪大学, 生命機能研究科, 准教授 (90263334)
• Project Period (FY): 2011-04-28 – 2015-03-31
• Keywords: 発現制御 / 遺伝子 / ゲノム / 細胞分化 / 転写制御因子

Outline of Final Research Achievements

Transcription factors function as heterologous complexes, and changing their partner in the complex results in a major alteration in their regulatory targets. This mechanism is responsible for the switching of cell states during differentiation. In addition, the DNA-binding sequences of transcription factor complexes are not mere additions of sequences for individual factor binding.
In this study, we systematically analyzed in vitro and in vivo DNA-binding sequences for Sox2-partner factor complexes. (1) We collected high-affinity Sox2;Pax6 co-binding sequences in vitro using a new SELEX procedure with non-RI EMSA and characterized these sequences. (2) We improved the ChIP-seq procedure using biotinylated transcription factors and applied it to epiblast stem cells to characterize Sox2;partner (e.g., Pou5f1) co-binding sequences in vivo.

Free Research Field

分子発生生物学