2012 Fiscal Year Final Research Report

Functional analysis of natural genetic variation of non-model species using model species

Research Project

Project/Area Number	23657015
Research Category	Grant-in-Aid for Challenging Exploratory Research
Allocation Type	Multi-year Fund
Research Field	Ecology/Environment
Research Institution	National Museum of Nature and Science, Tokyo
Principal Investigator	IKEDA Hajime 独立行政法人国立科学博物館, 植物研究部, 研究員 (70580405)
Project Period (FY)	2011 – 2012
Keywords	分子生態
Research Abstract	I have focused PHYE in Cardamine nipponica that have two alleles distinguished by several amino acid replacements in wild populations. At first, I succeed cloning PHYE into a vector to induce protein synthesis in E. coli. In particular, I made chimeric constructions that exchanged some amino acid replacements between alleles. In addition, I made construct of PHYE to apply transformation of Arabidopsis thaliana. Because PHYE is a large gene (ca. 3600 bp in protein coding region), I took much time for cloning and failed to obtain sufficient data about functional differences of alleles of PHYE. Nevertheless, my progress could contribute future work to unravel functional differences of alleles found in wild populations.