Engineering of photosynthetic pigment system

2012 Fiscal Year Final Research Report

• Project/Area Number: 23657026
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Plant molecular biology/Plant physiology
• Research Institution: Hokkaido University
• Principal Investigator: TANAKA Ayumi 北海道大学, 低温科学研究所, 教授 (10197402)
• Project Period (FY): 2011 – 2012
• Keywords: クロロフィル / 葉緑体 / 光合成 / 機能改変 / シロイヌナズナ

Research Abstract

The light-harvesting apparatus is an interesting and usefu1 targetfor genetic modulation of photosynthesis, because it participates in the initial andessential steps of photosynthesis. We succeeded in altering the pigment arrangement inthe light-harvesting systems by introducing N-terminal truncated chlorophyllide aoxygenase, which catalyzes chlorophyll b biosynthesis, into Arabidopsis thaliana.Interestingly, thistransgenicplant showed the stay green phenotype and exhibited severephotodamage. Studies of this transgenic plant suggested the potential agriculturalapPlication of photosynthesis modification.

Research Products

• [Journal Article] Conversionof chlorophyllbto chlorophyll a precedesmagnesium dechelation for protectionagainst necrosis in Arabidopsis (2012)
  • Author(s): Shimoda Y, Ito H, Tanaka A
  • Journal Title: Plantjourna1 Volume: 72(3) Pages: 501-511
  • DOI: DOI:10.1111/j.1365-313X.2012.05095.x
  • Peer Reviewed
• [Journal Article] ChlorophyllbReductase Plays an EssentialRole in Maturation and Storability of Arabidopsis thaliana Seeds (2012)
  • Author(s): Nakajima S, Ito H, Tanaka R, Tanaka A
  • Journal Title: Physiology Volume: 160(1) Pages: 261-273
  • DOI: DOI:10.1104/pp.112.19688
  • Peer Reviewed
• [Journal Article] Chlorophyll metabolism inphotosynthetic organisms. Handbook ofPorphyrin Science, edited by Kar1 M Kadish,Kevin M Smith and Roger Guilard (2012)
  • Author(s): Tanaka R, Takabayashi A, Ito H andTanaka A
  • Journal Title: WorldScientific, Singapore Volume: 20 Pages: 213-242
  • DOI: doi:10.1142/9789814335508_fmatterO5
  • Peer Reviewed
• [Journal Article] クロロフィル合成系の多様性はいかにして生まれたか (2012)
  • Author(s): 伊藤寿、堕巨歩、田中亮一
  • Journal Title: 光合成研究 Volume: 22(2) Pages: 98-105
  • URL: http://photosyn.jp/journa1/sections/kaiho64-6
  • Peer Reviewed
• [Journal Article] Overproduction of chlorophyll b retardssenescence through transcriptionalre-programming in Arabidopsis (2012)
  • Author(s): Sakuraba Y, Balazadeh S, Tanaka R,Mueller-Roeber B and Tanaka A
  • Journal Title: Plant CellPhysio1 Volume: 53(3) Pages: 505-517
  • DOI: DOI:10.1093/pcp/pcsOO6
  • Peer Reviewed
• [Journal Article] Luminescence of singletoxygen in photosystem II complexesisolated from cyanobacteriumSynechocystis sp. PCC6803 containingmonovinyl or divinyl chlorophyll (2012)
  • Author(s): Tomo, T.,Kusakabe, H.,Nagao, R., Ito,H.,工anaka, A.,Akimoto, S.,Mimuro, M. andOkazaki, S
  • Journal Title: Biochim Biophys Acta Volume: 1817(8) Pages: 1299-1305
  • DOI: DOI:10.1016/j.bbabio.2012.02.018
  • Peer Reviewed
• [Journal Article] Alterations in photosynthetic pigmentsand amino acid composition of Dl proteinchange energy distribution in photosystemII (2012)
  • Author(s): Yokono, M., Tomo, T., Nagao, R., Ito,H., Tanaka, A.. and Akimoto, S
  • Journal Title: Biochim Biophys Acta Volume: 1817(5) Pages: 754-759
  • DOI: DOI:10.1016/j.bbabio.2012.02.009
  • Peer Reviewed
• [Journal Article] Evolution ofa divinyl chlorophy11-based photosystemin Prochlorococcus (2011)
  • Author(s): Ito, H. and Tanaka, A
  • Journal Title: Proc Natl Acad Sci USA10 Volume: 8(44) Pages: 18014-18019
  • DOI: DOI:10.1073/pnas.1107590108
  • Peer Reviewed
• [Journal Article] The Oligomeric States of Photosystemsand Light-harvesting Complexes in theChlorophyll b-1ess Mutant (2011)
  • Author(s): Takabayashi, A.,Kurihara, K.,Kuwano,M., Kasahara, Y., Tanaka, R. and_Tanaka_A
  • Journal Title: Plant Ce11 Physiol Volume: 52(12) Pages: 2103-2114
  • DOI: DOI:10.1093/pcp/pcr138
  • Peer Reviewed
• [Journal Article] AMonogalactosyldiacy191ycerol SynthaseFound in the Green Sulfur BacteriumChlorobaculum tepidum Reveals ImportantRoles for Galactolipids in Photosynthesis (2011)
  • Author(s): Masuda, S., Harada, J., Yokono, M.,Yuzawa, Y., Shimojima, M., Murofushi, K.,Tanaka, H., Masuda, H., Murakawa, M.,Haraguchi, T., Kondo, M., Nishimura, M.,Yuasa, H.,Noguchi, M.,Oh-oka, H.,Tanaka,A,Tamiaki, H. and Ohta, H
  • Journal Title: Plant Ce11 Volume: 23(7) Pages: 2644-2658
  • DOI: DOI:10.1105/tpc.111.085357
  • Peer Reviewed
• [Journal Article] Identificationof the 7-Hydroxymethyl Chlorophyll aReductase of the Chlorophyll Cycle inArabidopsis (2011)
  • Author(s): Meguro, M., Ito, H.,Takabayashi, A.,Tanaka, R. and工anaka, A
  • Journal Title: Plant Ce11 Volume: 23(9) Pages: 3442-3453
  • DOI: DOI:10.1105/tpc.111.089714
  • Peer Reviewed
• [Journal Article] Chlorophyll cycle regulates theconstruction and destruction of thelight-harvesting complexes (2011)
  • Author(s): Tanaka, R. and Tanaka, A
  • Journal Title: BiophysBiochim Acta Volume: 1807 Pages: 968-976
  • DOI: DOI:10.1016/j.bbabio.2011.01.002
  • Peer Reviewed
• [Journal Article] NYC4, the rice ortholog of ArabidopsisTHF1, is involved in the degradation ofchlorophy11-protein complexes during leafsenescence
  • Author(s): Yamatani H, Sato Y, Masuda Y, Kato Y,Morita R, Fukunaga K, Nagamura Y,Nishimura M, Sakamoto W, Tanaka A, KusabaK
  • Journal Title: Plant journa1 Volume: (in press)
  • DOI: DOI:10.1111/tpj.12154
  • Peer Reviewed
• [Presentation] Possibleinvolvement of chlorophyllase in a plantdefense system (2013)
  • Author(s): Xueyun Hu, Masanori Oshiai, TohruTsuchiya, Stefan Hortensteiner, Ayumi Tanaka, Ryouichi Tanaka
  • Organizer: 第54回日本植物生理学会
  • Place of Presentation: 岡山大学、岡山市
  • Year and Date: 2013-03-22
• [Presentation] シロイヌナズナにおけるlight-harvesting-likeタンパク質LIL8の機能解析 (2013)
  • Author(s): 加藤由佳子、高林厚史、田中歩、田中亮一
  • Organizer: 第54回日本植物生理学会
  • Place of Presentation: 岡山大学、岡山市
  • Year and Date: 2013-03-22
• [Presentation] シロイヌナズナにおけるlight-harvesting-like protein, LIL3の機能解析 (2013)
  • Author(s): 高橋香織、高林厚史、田中歩、田中亮一
  • Organizer: 第54回日本植物生理学会
  • Place of Presentation: 岡山大学、岡山市
  • Year and Date: 2013-03-22
• [Presentation] Chlorophyllide a oxygenase(CAO)のnegative feedback制御におけるAドメインの機能解析 (2013)
  • Author(s): 森恭一郎、高林厚史、田中歩
  • Organizer: 第54回日本植物生理学会
  • Place of Presentation: 岡山大学、岡山市
  • Year and Date: 2013-03-22
• [Presentation] シロイヌナズナのクロロフィルb還元酵素の強光適応における機能解析 (2013)
  • Author(s): 佐藤玲惟、伊藤寿、旦虫歩
  • Organizer: 第54回日本植物生理学会
  • Place of Presentation: 岡山大学、岡山市
  • Year and Date: 2013-03-22
• [Presentation] Degradation of chlorophyll and chlorophyll-protein complexes,Okavama Universitv (2012)
  • Author(s): Avumi Tanaka
  • Organizer: International Symposium Structure and Dynamics of Photosynthetic Systems
  • Place of Presentation: Okayama University,Okayama
  • Year and Date: 2012-10-22
• [Presentation] light-harvesting-like proteinによるgeranylgeranyl reductaseの安定化 (2012)
  • Author(s): 高橋香織、高林厚史、田中歩、田中亮一
  • Organizer: ドリコールおよびイソプレノイド研究会例会
  • Place of Presentation: 新潟大学、新潟市
  • Year and Date: 2012-09-29
• [Presentation] 緑藻の色素タンパク質複合体の生化学的手法を用いた網羅的解析 (2012)
  • Author(s): 功刀基、高林厚史、里虫歩
  • Organizer: 化学工学会第44回秋季大会
  • Place of Presentation: 東北大学、仙台市
  • Year and Date: 2012-09-20
• [Presentation] 珪藻のクロロフィル合成の解析 (2012)
  • Author(s): 伊藤寿、田中歩
  • Organizer: 日本藻類学会第36回大会
  • Place of Presentation: 北海道大学学術交流会館、札幌市
  • Year and Date: 2012-07-14
• [Presentation] クロロフィルb合成酵素CAOのユニークな分子進化と光合成の多様化 (2012)
  • Author(s): 功刀基、高林厚史、田中歩
  • Organizer: 第3回日本光合成学会年会
  • Place of Presentation: 東京工業大学すずかけキャンパス、横浜市
  • Year and Date: 2012-06-01
• [Presentation] Genetic engineering ofphotosystems by the modification ofchlorophyll metabolism (2012)
  • Author(s): Ayumi Tanaka
  • Organizer: ABRC AcademiaSinica seminar
  • Place of Presentation: Instituteof Planr and Microbial Biology, Taipei,Taiwan
  • Year and Date: 2012-04-23
• [Presentation] Chlorophyllasel isnot involved inchlorophyll breakdown duringmethyl-jasmonate-induced senescence inArabidopsis thaliana (2012)
  • Author(s): Xueyun Hu, Tohru Tsuchiya, StefanHoertensteiner, Ayumi Tanaka, RyouichiTanaka
  • Organizer: 第53回日本植物生理学会年会
  • Place of Presentation: 京都産業大学、京都市
  • Year and Date: 2012-03-18
• [Presentation] BN-PAGEにより精製された光合成反応中心の時間分解蛍光スペクトルの測定 (2012)
  • Author(s): 横野牧生,高林厚史,栗原克宜,田中歩,秋本誠志
  • Organizer: 第53回日本植物生理学会年会
  • Place of Presentation: 京都産業大学、京都市
  • Year and Date: 2012-03-18
• [Presentation] DivinylChlorophyllide Reductaseの性質 (2012)
  • Author(s): 伊藤寿、田中歩
  • Organizer: 第53回日本植物生理学会年会
  • Place of Presentation: 京都産業大学、京都市
  • Year and Date: 2012-03-18
• [Presentation] 新規手法による葉緑体タンパク質複合体の網羅的検出 (2012)
  • Author(s): 高林厚史,栗原克宜,田中亮一,田中歩
  • Organizer: 第53回日本植物生理学会年会
  • Place of Presentation: 京都産業大学、京都市
  • Year and Date: 2012-03-18
• [Presentation] シロイヌナズナにおける新規Light-harvesting-likeタンパク質の機能解析 (2012)
  • Author(s): 加藤由佳子、高林厚史、里虫歩、田中亮一
  • Organizer: 第53回日本植物生理学会年会
  • Place of Presentation: 京都産業大学、京都市
  • Year and Date: 2012-03-16
• [Presentation] Light-harvesting chlorophyll-bindingproteinと共通するモチーフを持つタンパク質、LIL3の機能解析 (2012)
  • Author(s): 高橋香織、高林厚史、田中歩、田中亮一
  • Organizer: 第53回日本植物生理学会年会
  • Place of Presentation: 京都産業大学、京都市
  • Year and Date: 2012-03-16
• [Presentation] シロイヌナズナのクロロフィルb分解における複数経路の検証 (2012)
  • Author(s): 下田洋輔、伊藤寿、田中歩
  • Organizer: 第53回日本植物生理学会年会
  • Place of Presentation: 京都産業大学、京都市
  • Year and Date: 2012-03-16
• [Presentation] Evolution of chlorophyll metabolism (2011)
  • Author(s): Ayumi Tanaka
  • Organizer: The Evolutionof Photosynthesis and Oxygenation of theEarth
  • Place of Presentation: The Universityof New South Wales, Sydney, Australia
  • Year and Date: 20110628-29
• [Presentation] 生体金属イオン生命と分子をつなぐメッセンジャー (2011)
  • Author(s): 田中歩
  • Organizer: 第84回日本生化学会大会シンポジウム
  • Place of Presentation: 国立京都国際会館、京都市
  • Year and Date: 2011-09-21
• [Remarks]
  • URL: http://www.10wtem.hokudai.ac.jp/Plantadapt/index.htm1