Creation of artificial luminescent plant induced by luciferin biosynthesis system

2012 Fiscal Year Final Research Report

• Project/Area Number: 23657045
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Plant molecular biology/Plant physiology
• Research Institution: National Institute of Advanced Industrial Science and Technology
• Principal Investigator: OHMIYA Yoshihiro 独立行政法人産業技術総合研究所, バイオメディカル研究部門, 研究部門長 (20223951)
• Co-Investigator(Kenkyū-buntansha): MITSUDA Nobutaka 産業技術総合研究所, 生物プロセス研究部門, 主任研究員 (80450667)
• Project Period (FY): 2011 – 2012
• Keywords: 組み換え植物 / ルシフェリン / ルシフェラーゼ / イメージング / 有用物質生産

Research Abstract

To establish the bioluminescent plants, we transfect the luciferase genes and the luciferin synthesis related genes to plants. At the first step, we established the firefly luciferase gene expressed and Cypridina luciferase expressed plants and confirmed

Research Products

• [Journal Article] In Vivo Imaging Demonstrates ATP Release from Murine Keratinocytes and Its Involvement in Cutaneous Inflammation after Tape Stripping
  • Author(s): Takahashi T, Kimura Y, Niwa K, Ohmiya Y, Fujimura T, Yamasaki K, Aiba S
  • Journal Title: J Invest Dermatol Volume: (in press)
• [Journal Article] Interconversion of ketoprofen recognition in firefly luciferase-catalyzed enantioselective thioesterification reaction using from Pylocoeria miyako (PmL) and Hotaria parvura (HpL) just by mutating two amino acid residues
  • Author(s): Kato DI, Hiraishi Y, Maenaka M, Yokoyama K, Niwa K, Ohmiya Y, Takeo M, Negoro S
  • Journal Title: J Biotechnol Volume: (in press)
• [Presentation] Bioluminescence multicolor imaging using secreted and non-secreted luciferase (2013)
  • Author(s): OHMIYA YOSHIHIRO
  • Organizer: 組織培養学会年次発表会
  • Place of Presentation: 産総研共用行動(茨城県)
  • Year and Date: 2013-05-30