Comprehensive search for homologous sequences obtained from the Eukaryote with the unique DNA in the starfish sperm centrosome.

2014 Fiscal Year Final Research Report

• Project/Area Number: 23657067
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Biodiversity/Systematics
• Research Institution: Kanagawa University
• Principal Investigator: HINO Akiya 神奈川大学, 理学部, 教授 (00144113)
• Co-Investigator(Kenkyū-buntansha): KAWAI Shinobu 神奈川大学, 総合理学研究所, 客員研究員 (00409989) ; DEGAWA Yosuke 筑波大学, 生命環境科学研究科(系), 助教 (00311431)
• Project Period (FY): 2011-04-28 – 2015-03-31
• Keywords: centriole

Outline of Final Research Achievements

Recently, we isolated novel DNA from centrosomes in the sperm of the starfish Asterina pectinifera. Similar DNA sequences are also found other animal cells and the sperm of liverwort. In the present study, we examined whether this unique DNA contain in the Eukaryotic cells. We performed PCR analysis of total DNA from many Eukaryotic cells with Centrosome Spesfic primer. This pair of primers was designed to specifically amplify novel DNA in the starfish. In the case of Physarum polycephalum, we found that the size of PCR products were similar to those of starfish, i.e., approximately 500 bps. We determined nucleotide sequence similarities in the DNA of these PCR-amplified products of Amebozoa. The DNA sequences of P. polycephalum showed high homology (96.3% identity) with those obtained from the novel DNA. Our results show that Amebozoa have a conserved sequence that is similar to the novel DNA. This DNA is expected to be a new tool in the study of the origin of the eukaryotes.

Free Research Field

分子発生学