2012 Fiscal Year Final Research Report
Analysis of Interactions in High Molecular Weight Protein Complexes by Using Segment Label and Cutting-edge NMR Technologies
| Project/Area Number |
23657070
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Structural biochemistry
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| Research Institution | Hokkaido University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
UCHIDA Takeshi 北海道大学, 大学院・理学研究院, 助教 (30343742)
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| Project Period (FY) |
2011 – 2012
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| Keywords | セグメントラベル / 多次元NMR / 蛋白質複合体 / 蛋白質間相互作用 / 安定同位体ラベル |
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| Research Abstract |
To develop new methodologies for structural analysis of biologically important high-molecular-weight protein complexes, applications of the segment label and new NMR techniques such as the transfer cross-saturation (TCS) and residual dipole coupling (RDC) were examined. By using TCS for the final electron transfer complex in the respiratory chain, the complex between cytochrome c and cytochrome c oxidase, we successfully determined the amino acid residues of cytochrome c directly interacting with cytochrome c oxidase.
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