Development of ion-channel composed of nucleic acids

2012 Fiscal Year Final Research Report

• Project/Area Number: 23657072
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Structural biochemistry
• Research Institution: Kyoto University
• Principal Investigator: KATAHIRA Masato 京都大学, エネルギー理工学研究所, 教授 (70211844)
• Project Period (FY): 2011 – 2012
• Keywords: 核酸 / イオンチャネル / アプタマー / 4重鎖 / プリオン / リボザイム

Research Abstract

The structure of a DNA/RNA quadruplex resembles that of an ion-channel. We tested if the quadruplex functions as an ion-channel. The quadruplex was embedded into liposome and a flow of ions was examined using fluorescent indicators. The results suggested that the quadruplex could function as an ion-channel. We also succeeded to elucidate the structural basis of the quadruplex as an anti-prion agent. The quadruplex was also utilized to switch the activity of a ribozyme.

Research Products

• [Journal Article] Anti-prion ctivity of an RNA aptamer and its structural basis (2013)
  • Author(s): Mashima, T., Nishikawa, F., Kamatari, Y., Fujiwara, H., Saimura, M., Nagata, T., Kodaki, T., Nishikawa, S., Kuwata, K. and Katahira, M.
  • Journal Title: Nucleic Acids Res Volume: 41 Pages: 1355-1362
  • DOI: DOI:10.1093/nar/gks1132
  • Peer Reviewed
• [Journal Article] リボスイッチ及び抗プリオン活性を示す RNA アプタマーの動作メカニズム (2013)
  • Author(s): 片平正人
  • Journal Title: 実験医学、羊土社 Volume: Vol. 31 Pages: 68-74
  • URL: https://www.yodosha.co.jp/jikkenigaku/
• [Journal Article] Intelligent ribozyme that switches its activity in response to K^+ through quadruplex formation (2012)
  • Author(s): Nagata, T., Sakurai, Y., Hara, Y., Mashima, T., Kodaki, T. and Katahira, M.
  • Journal Title: FEBS J Volume: 279 Pages: 1456-1463
  • DOI: DOI:10.1111/j.1742-4658.2012.08538.x
  • Peer Reviewed
• [Journal Article] Structure of Musashi1 in a complex with target RNA: The role of aromatic stacking interactions (2012)
  • Author(s): Ohyama, T., Nagata, T., Tsuda, K., Kobayashi, N., Imai, T., Okano, H., Yamazaki, T. and Katahira, M.
  • Journal Title: Nucleic Acids Res Volume: 40 Pages: 3218-3231
  • DOI: DOI:10.1093/nar/gkr1139
  • Peer Reviewed
• [Journal Article] Structural aspects for the recognition of ATP by ribonucleopeptide receptors (2011)
  • Author(s): Nakano, S., Mashima, T., Matsugami, A., Inoue, M., Katahira, M. and Morii, T.
  • Journal Title: J. Am. Chem. Soc Volume: 133 Pages: 4567-4579
  • DOI: DOI:10.1021/ja110725d
  • Peer Reviewed
• [Presentation] Creation of Novl Ribozyme whose Activity Switches on in Response to K+ via Quadruples Formation (2012)
  • Author(s): Y. Yamaoki, T. Mashima, Y. Sakurai, Y. Hara, T. Kodaki, T. Nagata, M. Katahira
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡国際会議場(福岡市)
  • Year and Date: 2012-12-12
• [Presentation] Development of intelligent ribozyme whose activity switches on in response to K+via quadruplex formation (2012)
  • Author(s): Y. Yamaoki, T. Mashima, Y. Sakurai, Y. Hara, T. Nagata, M. Katahira
  • Organizer: The 39th Internaional Symposium on Nucleic Acids Chemistry
  • Place of Presentation: 名古屋大学(名古屋市)
  • Year and Date: 2012-11-16
• [Presentation] The 39th Internaional Symposium on Nucleic Acids Chemistry, Strctural analysisi of RNA aptamer in complex with prion protein, and its anti-prion activity (2012)
  • Author(s): T. Mashima, F, Nishikawa, Y. Kamatari, H. Fujiwara, T. Nagata, T. Kodaki, S. Nishikawa, K. Kuwata. M. Katahira
  • Organizer: The 39th Internaional Symposium on Nucleic Acids Chemistry
  • Place of Presentation: 名古屋大学(名古屋市)
  • Year and Date: 2012-11-15
• [Presentation] Anti-prion activity and its structural basis of RNA aptamer, and sliding-direction-dependent deaminase activity of anti-HIV enzyme (2012)
  • Author(s): Mashima. T., Furukawa, A., Sugase, K., Fujiwara, H., Nishikawa, F., Morishita, R., Ryo, A., Kamatari, Y., Nagata, T., Nishkawa, S., Kuwata, K. and Katahira, M.
  • Organizer: XXV International Conference on Magnetic Resonance in Biological Systems
  • Place of Presentation: Lyon (France)
  • Year and Date: 2012-08-21
• [Presentation] カリウムイオン濃度を感知して活性がオンになるインテリジェントリボザイムの創製 (2012)
  • Author(s): 山置雄大,真嶋司,櫻井佑,原ゆかり,永田崇,片平正人
  • Organizer: 第14回日本 RNA 学会年会
  • Place of Presentation: 東北大学(仙台市)
  • Year and Date: 2012-07-19
• [Presentation] Sliding-direction-dependent activity of anti-HIV enzyme,and structural basis of anti-prion activity of RNA aptamer (2012)
  • Author(s): A. Furukawa, T. Mashima, K. Sugase, H. Fujiwara, T. Nagata, R. Morishita, A. Ryo, F. Nishikawa, S. Nishikawa, Y. Kamatari, K. Kuwata, M. Katahira
  • Organizer: Korea-Japan Bilateral NMR Symposium
  • Place of Presentation: 北海道大学(札幌市)
  • Year and Date: 2012-03-16
• [Presentation] r(GGA)4 chimeric ribozyme switches its activity in responseto K+ via quadruplex formation (2011)
  • Author(s): T. Nagata, Y. Sakurai, Y. Hara, T. Mashima, M. Katahira
  • Organizer: The 38th International Symposium on Nucleic Acid Chemistry
  • Place of Presentation: 北海道大学(札幌市)
  • Year and Date: 2011-11-11
• [Presentation] Structural basis of the high affinity of the RNA aptamer against prion protein (2011)
  • Author(s): T. Mashima, H. Fujiwara, T. Koshida, M. Saimura, M. Imamura, T.Yokoyama, F. Nishikawa, S. Nisikawa, M. Katahira
  • Organizer: RNA2011 SIXTEEN ANNUAL MEETING OF THE RNA SOCIETY
  • Place of Presentation: 京都国際会議場(京都市)
  • Year and Date: 2011-06-15
• [Book] Encyclopedia of Biophysics, "Introduction to Nucleic Acids NMR" (2013)
  • Author(s): Katahira, M. and Mashima, T.
  • Total Pages: 1763-1772
  • Publisher: Springer.
• [Book] 日本化学会編・CSJ カレントレビュー"核酸化学のニュートレンド-DNA ・RNA の新たな可能性を開く" (分担)、Vol. 6 "論文にみる最重要概念と革新実験データ " (2011)
  • Author(s): 片平正人
  • Total Pages: 36-40
  • Publisher: 化学同人
• [Remarks]
  • URL: http://www.iae.kyoto-u.ac.jp/bio/