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2012 Fiscal Year Final Research Report

Specific host recognition mechanisms of marine virus, a dinoflagellate-infecting virus

Research Project

  • PDF
Project/Area Number 23657076
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Structural biochemistry
Research InstitutionUniversity of Miyazaki (2012)
Osaka University (2011)

Principal Investigator

WADA Kei  宮崎大学, テニュアトラック推進機構, 助教 (80379304)

Co-Investigator(Kenkyū-buntansha) FUKUYAMA Keiichi  大阪大学, 大学院・理学研究科, 教授 (80032283)
Project Period (FY) 2011 – 2012
Keywords分子認識および相互作用
Research Abstract

Heterosigma akashiwo virus (HaV) is a large virus specifically infecting the harmful bloom-forming flagellate Heterosigma akashiwo. Based on field surveys and cross-reactivity tests between the host clones and HaV clones, suggesting that this strain-dependent infection may play an important role in determining the clonal composition and maintaining the intraspecies diversity of natural H. akashiwo populations. In this study, to elucidate the mechanism of the specific host infection, the Vp183 protein, the specific host recognition protein, was overproduced.

  • Research Products

    (15 results)

All 2013 2012 2011 Other

All Journal Article (4 results) (of which Peer Reviewed: 4 results) Presentation (10 results) Remarks (1 results)

  • [Journal Article] Wada, K., Kimura, K., Hasegawa, H.,Fukuyama., K and Nagasaki, K2013

    • Author(s)
      Wada, K., Kimura, K., Hasegawa, H.,Fukuyama., K and Nagasaki, K
    • Journal Title

      Microbes Environ

      Volume: 27 Pages: 483-489

    • Peer Reviewed
  • [Journal Article] hermal denaturation andrenaturation of γ-glutamyltranspeptidase of Escherichiacoli2013

    • Author(s)
      Ho T.V, Kamei, K. Wada, K., Fukuyama,K. and Suzuki, H
    • Journal Title

      Biosci. Biotechnol. Biochem

      Volume: 77 Pages: 409-4012

    • Peer Reviewed
  • [Journal Article] large-scale overexpression methodof deriving carbon monoxide dehydrogenaseII from the thermophilic bacteriumCarboxdothermus hydrpgenformans2011

    • Author(s)
      Inoue, T., Yoshida, Y., Wada, K.,Daifuku, T., Fukuyama, K., Sako, Y. Asimple
    • Journal Title

      Biosci.Biotech. Biochem.

      Volume: 75 Pages: 1382-1394

    • Peer Reviewed
  • [Journal Article] Mapping of protein-proteininteraction sites in the plant-type[2Fe-2S] ferredoxin2011

    • Author(s)
      Kameda, H., Hirabayashi, K., Wada,K.,Fukuyama K
    • Journal Title

      PLoS ONE

      Pages: e21947

    • Peer Reviewed
  • [Presentation] Preliminary X-raydiffraction analysis and establishment ofthe immunoassay platform of thedinoflagellate-infecting marine virusHcRNAV.2012

    • Author(s)
      Tanaka, E, Hasegawa, A., Kimura, K.,Nakaniwa, T., Tomaru, Y, Fukuyama, K.,Nagasaki, K., Wada, K
    • Organizer
      The Asian Crystallographic Society and theSociety of Crystallographers in Australiaand New Zealand
    • Place of Presentation
      Adelaide Convention Centre(オーストラリア アデレード)
    • Year and Date
      20121202-05
  • [Presentation] 有害赤潮原因藻に感染するウイルスHcRNAV粒子の結晶学的研究および特異的抗体の性状解析2012

    • Author(s)
      長谷川昭文、木村圭、外丸裕司、長崎慶三、福山恵一、和田啓
    • Organizer
      日本結晶学会平成24年度年会
    • Place of Presentation
      東北大学片平キャンパス
    • Year and Date
      20121025-26
  • [Presentation] 有害赤潮原因藻に感染するウイルスHcRNAVの精製と結晶化2012

    • Author(s)
      長谷川昭文、木村圭、外丸裕司、福山恵一、長崎慶三、和田啓
    • Organizer
      第12回蛋白質科学会年会
    • Place of Presentation
      名古屋国際会議場
    • Year and Date
      20120620-22
  • [Presentation] 有害渦鞭毛藻ヘテロカプサに感染する HcRNAV を検出する抗体の開発と実用性の検証2012

    • Author(s)
      木村 圭、和田 啓、長谷川昭文、福山恵一、外丸裕司、長崎慶三
    • Organizer
      成 24 年度日本水産学会春季大会
    • Place of Presentation
      東京海洋大学品川キャンパス
    • Year and Date
      2012-03-29
  • [Presentation] Structural biology of the SUFmachinery involved in de novo iron-sulfurcluster biosynthesis.2012

    • Author(s)
      Wada K
    • Organizer
      4th FEBS SpecialMeeting, ATP-Binding Cassette Proteins:From Multidrug Registance to GeneticDiseases
    • Place of Presentation
      Hotel Grauer Bar(オーストリア インスブルック)
    • Year and Date
      2012-03-04
  • [Presentation] 有害赤潮原因藻に感染するウイルスHcRNAV粒子の結晶学的研究2011

    • Author(s)
      長谷川昭文、田中悠喜、外丸裕司、長崎慶三、福山恵一、和田啓
    • Organizer
      第61回日本結晶学会年会
    • Place of Presentation
      北海道大学
    • Year and Date
      2011-11-24
  • [Presentation] フェレドキシン依存的ビリン還元酵素PcyA変異体-ビリベルジン複合体の構造解析2011

    • Author(s)
      和田啓、萩原義徳、入川鉄平、油谷裕子、福山恵一
    • Organizer
      第84回日本生化学会大会
    • Place of Presentation
      国立京都国際会館
    • Year and Date
      2011-09-22
  • [Presentation] Oneresidue substitution in PcyA leads tounexpected changes in tetrapyrrolesubstrate binding2011

    • Author(s)
      Wada K., Hagiwara Y., Fukuyama, K
    • Organizer
      XXII Congress andGeneral Assembly International Union ofCrystallography
    • Place of Presentation
      Palacio Municipal de Madrid(スペイン マドリード)
    • Year and Date
      2011-08-27
  • [Presentation] Crystallographicanalyses of the ISC proteins involved in denovo Fe-S cluster biogenesis2011

    • Author(s)
      Hirabayashi, K., Takahashi, Y.,Fukuyama, K., Wada, K
    • Organizer
      XXIICongress and General AssemblyInternational Union of Crystallography
    • Place of Presentation
      Palacio Municipal deMadrid(スペイン マドリード)
    • Year and Date
      2011-08-27
  • [Presentation] 超好熱菌Aquifex aeolicusの鉄硫黄クラスター生合成に関わる脱硫黄酵素IscSの構造解析2011

    • Author(s)
      平林佳、岩永朋子、高橋康弘、福山恵一、和田啓
    • Organizer
      第11回日本蛋白質科学会年会
    • Place of Presentation
      ホテル阪急エキスポパーク
    • Year and Date
      2011-06-07
  • [Remarks]

    • URL

      http://www.cc.miyazaki-u.ac.jp/keiwada/index.htm

URL: 

Published: 2014-09-25  

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