2012 Fiscal Year Final Research Report
Roles of degradation products generated by the proteasome
| Project/Area Number |
23657083
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Functional biochemistry
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| Research Institution | The University of Tokyo |
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Principal Investigator |
MURATA Shigeo 東京大学, 大学院・薬学系研究科, 教授 (20344070)
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| Project Period (FY) |
2011 – 2012
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| Keywords | 細胞内タンパク質分解 |
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| Research Abstract |
The proteasome is the major proteolytic enzyme and irreversibly inactivate protein functions in cells. We assumed that short peptides generated by proteasomal degradation of cellular proteins have some physiological functions. To examine this hypothesis, we purified cytosolic and nuclear short peptides with C18-column. By using mass spectrometry, we succeeded in identifying sequences of these peptides. We also started to generate cell lines and mice in which peptide cleavage bythe proteasome is altered.
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[Journal Article] Decreased proteasomal activity causes age-related phenotypes and promotes the development of metabolic abnormalities.2012
Author(s)
Tomaru U, Takahashi S, Ishizu A, Miyatake Y, Gohda A, Suzuki S, Ono A, Ohara J, Baba T, Murata S, Tanaka K, Kasahara M.
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Journal Title
Am J Pathol.
Volume: 180(3)
Pages: 963-72
DOI
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[Journal Article] A mutation in the immunoproteasome subunit PSMB8 causes autoinflammation and lipodystrophy in humans.2011
Author(s)
Kitamura A, Maekawa Y, Uehara H, Izumi K, Kawachi I, Nishizawa M, Toyoshima Y, Takahashi H, Standley DM, Tanaka K, Hamazaki J, Murata S, Obara K, Toyoshima I, Yasutomo K.
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Journal Title
J Clin Invest.
Volume: 121(10)
Pages: 4150-60
DOI
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[Journal Article] Proteasome assembly defect due to a proteasome subunit beta type 8 (PSMB8) mutation causes the autoinflammatory disorder,Nakajo-Nishimura syndrome.2011
Author(s)
Arima K, Kinoshita A, Mishima H, Kanazawa N, Kaneko T, Mizushima T, Ichinose K, Nakamura H, Tsujino A, Kawakami A, Matsunaka M, Kasagi S,Kawano S, Kumagai S, Ohmura K, Mimori T, Hirano M, Ueno S, Tanaka K, Tanaka M, Toyoshima I, Sugino H, Yamakawa A, Tanaka K, Niikawa N, Furukawa F, Murata S, Eguchi K, Ida H, Yoshiura K.
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Journal Title
Proc Natl Acad Sci U S A.
Volume: 108(36)
Pages: 14914-9
DOI
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