Development of oriented reconstitution methods for membrane proteins

2012 Fiscal Year Final Research Report

• Project/Area Number: 23657091
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Functional biochemistry
• Research Institution: Okayama University
• Principal Investigator: OMOTE Hiroshi 岡山大学, 大学院・医歯薬学総合研究科, 准教授 (10273707)
• Project Period (FY): 2011 – 2012
• Keywords: 生体膜 / トランスポーター / タンパク質 / 生化学 / 神経 / 輸送

Research Abstract

Transporters contribute to various biological activity including uptake of nutrients, exclusion of waste products and drugs. It also contribute to signal transmission through their transport activity across the membrane. Measurement of transport activity is an essential part of functional study of transporters and development of novel drugs. In this study, we investigated the transport assay with reconstituted liposomes.

Research Products

• [Journal Article] 塩素イオンにより制御されるトランスポーター群の発見と意義 (2013)
  • Author(s): 表 弘志、森山芳則
  • Journal Title: 医学の歩み Volume: 245 Pages: 69-74
• [Journal Article] Vesicular Neurotransmitter Transporters: An approach for studying transporters with purified proteins (2012)
  • Author(s): Omote, H., *Moriyama, Y.
  • Journal Title: Physiology Volume: 28 Pages: 39-50
  • DOI: doi:10.1152/physiol.00033.2012.
• [Journal Article] A Na+-phosphate cotransporter homologue SLC17A4 protein) is an intestinal organic anion exporter. (2012)
  • Author(s): Togawa N, Miyaji, T,Izawa S,Omote, H.,*Moriyama Y.
  • Journal Title: Am J Physiol Cell Physiol. Volume: 302 Pages: C1652-C1660
  • DOI: doi:10.1152/ajpcell. 00015.2012.
• [Journal Article] Divalent cation transport by vesicular nucleotide transporter. (2011)
  • Author(s): Miyaji, T, Sawada K.Omote, H.,*Moriyama, Y.
  • Journal Title: J. Biol. Chem. Volume: 286 Pages: 42881-7
  • DOI: doi:10.1074/jbc.M111.277269
• [Journal Article] Functional characterization of vesicular excitatory amino acid transport by human sialin. (2011)
  • Author(s): Miyaji, T, Omote, H. *Moriyama, Y.
  • Journal Title: J. Neurochem Volume: 119 Pages: 1-5
  • DOI: doi: 10.1111/j.1471-4159.2011.07388.x.
• [Journal Article] Vesicular neurotransmitter transporter: bioenergetics and regulation of glutamate transport. (2011)
  • Author(s): Omote, H. Miyaji, T, Juge, N. and Moriyama, Y.
  • Journal Title: Biochemistry Volume: 50 Pages: 5558-65
  • DOI: doi:10.1021/bi200 k
• [Journal Article] Characterization of he human MATE2 proton-coupled polyspecific organic cation exporter. (2011)
  • Author(s): Komatsu, T., Hiasa, M., Miyaji, T.,Kanamoto, T., Matsumoto, T., Otsuka, M.,Moriyama, Y. and *Omote, H
  • Journal Title: In.t J. Biochtem. Cell Biol Volume: 43 Pages: 913-8
  • DOI: doi:10.1016/j.biocel.2011.03.005.
• [Presentation] NPTホモログは新トランスポーターである (2012)
  • Author(s): 表 弘志
  • Organizer: 日本生体エネルギー研究会 第38回討論会
  • Place of Presentation: 岡山
  • Year and Date: 20121222-24
• [Presentation] NPTホモログは新規尿酸排出トランスポーターである (2012)
  • Author(s): 表 弘志
  • Organizer: 生体膜と薬物の相互作用シンポジウム
  • Place of Presentation: 京都
  • Year and Date: 20121115-16
• [Presentation] 小胞型グルタミン酸トランスポーターの活性制御 (2011)
  • Author(s): 表 弘志
  • Organizer: 日本生体エネルギー研究会 第36回討論会
  • Place of Presentation: 京都
  • Year and Date: 20111220-22
• [Presentation] 小胞型神経伝達物質トランスポーターの機能制御 (2011)
  • Author(s): 表 弘志
  • Organizer: 生体膜と薬物の相互作用シンポジウム
  • Place of Presentation: 岡山
  • Year and Date: 20111124-25
• [Remarks]
  • URL: http://www.pharm.okayama-u.ac.jp/lab/seika/home/index.html