2012 Fiscal Year Final Research Report
Novel functions of ASF/SF2 in somatic hypermutation on the immunoglobulin gene.
| Project/Area Number |
23657092
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Functional biochemistry
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| Research Institution | Okayama University |
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Principal Investigator |
KANAYAMA Naoki 岡山大学, 大学院・自然科学研究科, 准教授 (70304334)
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| Project Period (FY) |
2011 – 2012
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| Keywords | 抗体 / 親和性成熟 / 体細胞高頻度突然変異 / スプライシング因子 |
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| Research Abstract |
The affinity of antibodies for antigens is improved by somatic hypermutation (SHM) occurred on the immunoglobulin (Ig) gene. In this study, using the hypermutating chicken B cell line DT40, we found that an isoform of the alternative-splicing factor ASF/SF2, ASF3 is essential for SHM on the Ig gene. The results also suggest that ASF3 might have a role in the activation and targeting of the mutation machinery through the regulation of post-transcriptional processing on the Ig gene.
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[Journal Article] Activation-induced cytidine deaminase (AID)-dependent somatic hypermutation requires a splice isoform of the serine/arginine-rich (SR) protein SRSF12012
Author(s)
Yuichi Kanehiro, Kagefumi Todo, Misaki Negishi, Junji Fukuoka, Wenjian Gan, Takuya Hikasa, Yoshiaki Kaga, Masayuki Takemoto, Masaki Magari, Xialu Li, James L. Manley, Hitoshi Ohmori, Naoki Kanayama
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Journal Title
Proceedings of the National Academy of Science of the United States of America
Volume: 109
Pages: 1216-21
DOI
Peer Reviewed
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