2012 Fiscal Year Final Research Report
Regulation cell function by light using LOV photoreceptive module
| Project/Area Number |
23657105
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Biophysics
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| Research Institution | Osaka Prefecture University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
IHARA Hideshi 大阪府立大学, 大学院・理学系研究科, 准教授 (60254447)
OKAJIMA Koji 大阪府立大学, 大学院・理学系研究科, 特認助教 (20438245)
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| Project Period (FY) |
2011 – 2012
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| Keywords | フォトトロピン / LOV ドメイン / MAP キナーゼ / キナーゼ活性光制御 |
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| Research Abstract |
Phototropin is a blue light sensor in plant which mediates phototropic response et al., and act as a blue light regulated protein kinase. Using the photoactivation mechanism of kinase in the phototropin, we aim to produce a new animal cell of which physiological responses, cell division and cell differentiation, can be switchable by light illumination through controlling the kinase activity of the key protein kinase involved. We introduced the in vitro analysis system in which the regulation of the key enzyme activity can be studied.
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[Journal Article] Mutations in the N-terminal flanking region of the blue-light sensing domain LOV2 disrupt itsrepressive activity on the kinase domain in the Chlamydomonas phototropin2012
Author(s)
Aihara, Y., Yamamoto, T., Okajima, K.,Yamamoto, K., Suzuki, T., Tokutomi, S., Tanaka,K. and Nagatani, A
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Journal Title
J. Biol.Chem
Volume: Vol.287
Pages: 9901-9909
Peer Reviewed
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[Journal Article] Light-Induced movement of the LOV2 domain in an Asp720Asn mutant LOV2-Kinase fragment of Arabidopsis phototropin 22011
Author(s)
Takayama, Y., Nakasako, M., Okajima,K., Iwata, A., Kashojiya, S., Matsui, Y. and Tokutomi, S
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Journal Title
Biochemistry
Volume: Vol. 50
Pages: 1174-1183
Peer Reviewed
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