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2012 Fiscal Year Final Research Report

Development of temperature sensing fluorescent protein for in vivo thermal imaging induced by infrared laser irradiation to cells

Research Project

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Project/Area Number 23657109
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Biophysics
Research InstitutionNational Institute for Basic Biology

Principal Investigator

KAMEI Yasuhiro  基礎生物学研究所, 生物機能解析センター, 特任准教授 (70372563)

Project Period (FY) 2011 – 2012
Keywordsバイオイメージング / 遺伝子発現 / 蛍光タンパク質 / 赤外レーザー
Research Abstract

Single-cell gene induction is a powerful method for gene function and cell fate analysis. Infrared laser evoked gene operator method can enable this by utilizing heat shock response system to induce a target gene in the single cell of transgenic animals or plants which carries the target gene driven by heat shock promoter. However, because excess heating results in cell damage, we developed temperature-sensitive fluorescent protein and real time temperature sensing microscope system to improve the method

  • Research Products

    (15 results)

All 2013 2012 2011 Other

All Journal Article (4 results) (of which Peer Reviewed: 4 results) Presentation (6 results) Book (1 results) Remarks (4 results)

  • [Journal Article] Trunk exoskeleton in teleosts is mesodermal in origin2013

    • Author(s)
      Shimada A, Kawanishi T, Kaneko T,Yoshihara H, Yano T, Inohaya K, Kinoshita M, Kamei Y, Tamura K and Takeda H
    • Journal Title

      Nature Communications

      Volume: 4 Pages: 1639

    • DOI

      doi:10.1038/ncomms2643

    • Peer Reviewed
  • [Journal Article] A heat-inducible CRE/LOXP gene induction system in medaka2013

    • Author(s)
      Kobayashi K, Kamei Y, Kinoshita M, Czerny T, Tanaka M
    • Journal Title

      Genesis

      Volume: 51 Pages: 59-67

    • Peer Reviewed
  • [Journal Article] Application of infrared laser to the zebrafish vascular system: gene induction, tracing, and ablation of single endothelal cells

    • Author(s)
      Kimura E, Deguchi T, Kamei Y, Shoji W, Yuba S and Hitomi J
    • Journal Title

      Arterioscler. Thromb. Vasc. Biol

    • Peer Reviewed
  • [Journal Article] Controlled Cre/loxP site-specific recombination in the developing brain in medaka fish, Oryzias latipes

    • Author(s)
      Okuyama T, Isoe Y, Hoki M, Suehiro Y,Yamagishi G, Naruse K, Kinoshita M, Kamei Y, Shimizu A, Kubo T and Takeuchi H
    • Journal Title

      PLoS One

    • Peer Reviewed
  • [Presentation] Applications of IR-LEGO (infrared laser-evoked gene operator) to the model plant as collaborative research projects in NIBB2013

    • Author(s)
      亀井保博(他9名)
    • Organizer
      日本植物生理学会
    • Place of Presentation
      岡山大学(岡山県)
    • Year and Date
      2013-03-22
  • [Presentation] Infrared Laser Mediated Single-Cell Gene Induction Method: Development and Application of IR-LEGO System2012

    • Author(s)
      Misako Saida-Taniguchi, Takuya Kaneko, Atsuko Shimada, Hiroyuki Takeda and Yasuhiro Kamei
    • Organizer
      18th Japanese Medaka and Zebrafish Meeting
    • Place of Presentation
      京都大学 (京都 府)
    • Year and Date
      2012-09-22
  • [Presentation] Development of IR laser-mediated gene induction system2012

    • Author(s)
      Yasuhiro Kamei
    • Organizer
      2^<nd> NUS-TLL-NIBB International Practical Course-Genetics, Genomics and Imaging in Zebrafish and Medaka
    • Place of Presentation
      National Univ. of Singapore (シンガポール)招待講演
    • Year and Date
      2012-07-26
  • [Presentation] 赤外レーザーによる1 細胞遺伝子発現法2012

    • Author(s)
      亀井保博, 浦和博子, 岡田清孝
    • Organizer
      日本植物生理学会
    • Place of Presentation
      京都産業大学(京都府)
    • Year and Date
      2012-03-16
  • [Presentation] 赤外レーザーによる遺伝子発現システム(IR-LEGO)の様々 なモデル生物への応用2011

    • Author(s)
      亀井保博,(他16名)
    • Organizer
      日本分子生物学会
    • Place of Presentation
      パシフィコ横浜 (神奈川県)
    • Year and Date
      2011-12-13
  • [Presentation] Infrared laser mediated single-cell gene induction method: Development and application of IR-LEGO system. 1st Strategic meeting for medaka research method: Development and application of IR-LEGO system2011

    • Author(s)
      Yasuhiro Kamei
    • Organizer
      1^<st> Strategic meeting for medaka research
    • Place of Presentation
      岡崎カンファレンスセンター(愛知県)
    • Year and Date
      2011-11-23
  • [Book] 生物機能モデルと新しいリソース・リサーチツール2011

    • Author(s)
      亀井保博(他111 名)
    • Publisher
      エルアイシー出版
  • [Remarks] ホームページ 研究内容ならびに研究成果は研究室HPに随時掲載

    • URL

      http://www.nibb.ac.jp/lspectro/kamei.htm#original

  • [Remarks] プレスリリース Nature Communications 誌での論文発表に 関しては東京大学ならびに基礎生物学研究所からプレスリリースを実施(2013 年3 月 28 日)。

  • [Remarks] Arterioscler. Thromb. Vasc. Biol.誌での論文発表に関しては岩手医科大学・基礎生物学研究所共同プレスリリースを実施(2013年5月31日予定)。

  • [Remarks] 技術講習会局所遺伝子発現技術(IR-LEGO 法)はトレーニングコースとして、シンガポール大学 (2012年7月)と、基礎生物学研究所(2012年10月)で実施した。また、2013年6月に中部地区技術職員向けにも実施予定。

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Published: 2014-09-25  

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