2012 Fiscal Year Final Research Report
The analysis of cell-to-cell communications by re-constructive artificial networks of neurons using non invasive cell collection techniques
| Project/Area Number |
23657111
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Biophysics
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| Research Institution | 公益財団法人神奈川科学技術アカデミー (2012)
Kanagawa Academy of Science and Technology (2011) |
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Principal Investigator |
TERAZONO Hideyuki 公益財団法人神奈川科学技術アカデミー, オンチップ・セロミクスプロジェクト, 研究員 (30398143)
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| Project Period (FY) |
2011 – 2012
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| Keywords | 脳 / 神経系の情報処理/ 細胞間コミュニケーション |
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| Research Abstract |
We have been studying the living system by assembling artificial tissues composed of single cells as a minimum functional unit. Along this research theme, we have studied cell-to-cell communications by controlling the spatial pattern of cell networks. In the course of research, our purpose is to understand information processing of neuron networks. In the results, we developed a non-invasive culturing and cell sorting method for primary neurons using alginate thin sheet. Usually, neurons cannot be collected selectively because neurons form spherical so that cannot be distinguished by floating condition.Moreover, after growth, if there are injured by protease, neurons will be died. The method we developed allows each single particular neuron to collect after axon or neurites are growth. By combine our method and the agarose micro-patterning techniques to make neuronal network artificially, neuronal networks using actual neurons can be constructed artificially and we will demonstrate the principle how neuronal networks work.
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