Degradation mechanisms of sigma 32 by membrane targeting via SRP pathway

2012 Fiscal Year Final Research Report

• Project/Area Number: 23657128
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Cell biology
• Research Institution: Kyoto University
• Principal Investigator: MORI Hiroyuki 京都大学, ウイルス研究所, 准教授 (10243271)
• Co-Investigator(Renkei-kenkyūsha): AKIYAMA Yoshinori 京都大学, ウイルス研究所, 教授 (10192460) ; ITO Koreaki 京都産業大学, 総合生命科学部, 教授 (90027334)
• Project Period (FY): 2011 – 2012
• Keywords: 生体膜

Research Abstract

In Escherichia coli.,a heat shock responsible transcription factor, sigma 32 is mainly and rapidly degraded by a membrane-bound ATP dependent protease, FtsH. However, precise molecular mechanisms of its degradation remain poorly understood. Using genetic analysis, Dr. Takashi Yura, one of our collaborators recently proposed that the SRP pathway involved in membrane protein biogenesis plays a significant role in the sigma 32 degradation. In this study, we carried out site-specific in vivo photo crosslinking analysis targeted to the sigma 32 and found that Ffh protein, a member of the SRP is in close proximity to the sigma 32.

Research Products

• [Journal Article] 細菌のタンパク質分泌を促進する膜タンパク質SecDF の構造と機能 (2013)
  • Author(s): 森 博幸、塚崎智也
  • Journal Title: 化学と生物 Volume: 51巻 Pages: 28-35
  • Peer Reviewed
• [Journal Article] PDZ domains of RseP are not essential for sequential cleavage of RseA or stress-induced σ^E activation in vivo. (2012)
  • Author(s): Hizukuri, Y. & Akiyama, Y.
  • Journal Title: Mol. Microbiol. Volume: 86 Pages: 1232-1245
  • Peer Reviewed
• [Journal Article] Structure and functionof a membrane component SecDF that enhances protein export. (2011)
  • Author(s): Tsukazaki, T.*, Mori, H.*, Echizen, Y., Ishitani, R., Fukai, S., Tanaka, T., Perederina, A., Vassylyev, D. G., Kohno, T.,Matsurana, A. D., Ito, K. &Nureki O.
  • Journal Title: Nature Volume: 474 Pages: 235-238
  • DOI: doi:10.1038/nature09980.
  • Peer Reviewed
• [Journal Article] Post liberation cleavage of signal peptides is catalyzed by the S2P protease in bacteria. (2011)
  • Author(s): Saito, A., Hizukuri, Y., Matsuo, E.-i., Chiba, S., Mori, H., Nishimura, O.,Ito, K., & Akiyama, Y.
  • Journal Title: Proc. Natl. Acad. Sci. USA Volume: 108 Pages: 13740-13745
  • DOI: doi:10.1073/pnas.1108376108.
  • Peer Reviewed
• [Journal Article] Nascentome analysis uncovers futile protein synthesis in Escherichia coli. (2011)
  • Author(s): Ito, K., Chadani, Y., Nakamori, K., Chiba, S., Akiyama, Y., &Abo, T.
  • Journal Title: PLoS ONE Volume: 6 Pages: e28413
  • Peer Reviewed
• [Journal Article] Recruitment of a species-specific translational arrest module to monitor different cellular processes. (2011)
  • Author(s): Chiba, S., Kanamori, T., Ueda, T., Akiyama, Y., Pogliano, K. & Ito, K.
  • Journal Title: Proc. Natl. Acad. Sci. USA. Volume: 108 Pages: 6073-6078
  • Peer Reviewed
• [Presentation] Structure and functionof SecDF, a membrane integrated protein translocation enhancing facor. (2013)
  • Author(s): 森 博幸、塚崎智也、町田裕紀子、三登一八、濡木理、伊藤維昭、秋山芳展
  • Organizer: 第86回日本細菌学会ワークショップ「細胞構造研究の新展開」
  • Place of Presentation: 千葉
  • Year and Date: 2013-03-19
• [Presentation] ビブリオ菌のタンパク質分泌マシナリー:SecDFパラログの発現制御機構 (2012)
  • Author(s): 森 博幸、橋本成祐、小嶋誠司、本間道夫、秋山芳展
  • Organizer: 第85回日本生化学会大会シンポジウム「運動超分子マシナリーの機能メカニズム」
  • Place of Presentation: 福岡
  • Year and Date: 2012-12-15
• [Presentation] 細菌の蛋白質膜透過装置の構造・機能・病原性との関わり (2011)
  • Author(s): 森 博幸
  • Organizer: 第48回細菌学会中部支部会(特別講演)名古屋
  • Place of Presentation: 名古屋
  • Year and Date: 20111021-22
• [Presentation] 蛋白質膜透過を促進する膜内在性因子SecDFの構造と機能 (2011)
  • Author(s): 森 博幸、塚崎智也、越前友香、秋山芳展、濡木 理、伊藤維昭
  • Organizer: 第84回日本生化学会大会シンポジウム「生体膜エネルギー変換装置の超分子科学」
  • Place of Presentation: 京都
  • Year and Date: 20110921-24
• [Presentation] プロトン駆動力を用いたSecDF による蛋白質膜透過の昂進機構 (2011)
  • Author(s): 森 博幸、塚崎智也、越前友香、濡木理、伊藤維昭
  • Organizer: 第11回日本蛋白質科学会年会ワークショップ「細胞膜ダイナミクス:In vitro再構成系によるアプローチ」
  • Place of Presentation: 大阪
  • Year and Date: 20110607-09
• [Remarks]
  • URL: http://www.virus.kyoto-u.ac.jp/Lab/akiyama/index.html