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2012 Fiscal Year Final Research Report

Exploitation of a tool for analyzing molecular interactions on the plasma membrane by using membrane-bound antibodies

Research Project

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Project/Area Number 23657142
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Developmental biology
Research InstitutionThe University of Tokyo

Principal Investigator

TAIRA Masanori  東京大学, 大学院・理学系研究科, 准教授 (60150083)

Project Period (FY) 2011 – 2012
Keywords膜結合型抗体 / 一本鎖抗体 / 細胞間架橋 / 力学的相互作用
Research Abstract

We cloned cDNAs for anti-HA and anti-Myc monoclonal antibodies, and made membrane-bound antibody constructs, which were verified to function as antibodies against HA- and Myc-tagged proteins. We further made single-stranded antibodies, which were verified to function as antibodies. Using membrane-bound antibodies, we next tried to measure the tension between two adjacent cells in Xenopus embryos by using the FRET-based tension sensor module (TSMod). In addition, we found that development of Xenopus gastrulae is affected when cells are bridged with the membrane-bound antibodies and membrane-bound antigens. This bridging method can be used as a tool for investigation of intercellular plasticity during morphogenesis.

  • Research Products

    (6 results)

All 2012 2011 Other

All Journal Article (2 results) Presentation (3 results) Remarks (1 results)

  • [Journal Article] Dynamic in vivo binding of transcription factors to cis-regulatory modules of cer and gsc in the stepwise formation of the Spemann-Mangold organizer.2012

    • Author(s)
      Sudou, N., Yamamoto, S., Ogino, H. and Taira, M.
    • Journal Title

      Development

      Volume: 139 Pages: 1651-1661

  • [Journal Article] The medaka enhancer mutant for zic1/zic4 provides molecular insights into teleost caudal fin evolution.2012

    • Author(s)
      Moriyama, Y., Kawanishi, T., Nakamura, R., Tsukahara, T., Sumiyama, K., Suster, M., Kawakami, K., Toyoda, A., Fujiyama, A., Yasuoka, Y., Nagao, Y., Sawatari, E., Shimikzu, A., Wakamatsu, Y., Hibi, M., Taira, M., Okabe, M., Naruse, K., Hashimoto, H., Shimada, A., Takeda, H.
    • Journal Title

      Curr. Biol.

      Volume: 22 Pages: 601-607

  • [Presentation] Heparan sulfate nanostructures regulate extracellular Wnt distribution and act asa core for Wnt/Dishevelled signalosome formation2012

    • Author(s)
      Masanori Taira and Yusuke Mii
    • Organizer
      14th International Xenopus Conference
    • Place of Presentation
      Giens Peninsula (France)
    • Year and Date
      20120909-13
  • [Presentation] Frzbregulates extracellular distribution of Wnt8 via interactions with heparan sulfate2011

    • Author(s)
      Yusuke Mii, Kenichi Nakazato, AtsushiMochizuki and Masanori Taira
    • Organizer
      Wnt 2011 Meeting, UCLA
    • Place of Presentation
      California, U.S.A.
    • Year and Date
      20110629-0703
  • [Presentation] Secreted Frizzled-related proteins (sFRPs) regulate extracellular distribution of Wnt ligands via interactions with heparan sulfate.(分泌性 Frizzled 関連蛋白質 (sFRPs) はヘパラン硫酸を介して細胞外の Wnt リガンドの分布を制御する。)2011

    • Author(s)
      Yusuke Mii, Kenichi Nakazato, Atsushi Mochizuki and Masanori Taira
    • Organizer
      日本発生生物学会第44回大会
    • Place of Presentation
      沖縄コンベンションセンター
    • Year and Date
      20110519-21
  • [Remarks]

    • URL

      http://www.biol.s.u-tokyo.ac.jp/users/lmb/index.php-Home

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Published: 2014-09-25  

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