2012 Fiscal Year Final Research Report
Establishment of a novel approach to select the best iPSCs without cell cloning
| Project/Area Number |
23657147
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Developmental biology
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| Research Institution | Kyoto University |
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Principal Investigator |
SAITO Megumu 京都大学, iPS 細胞研究所, 准教授 (90535486)
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| Project Period (FY) |
2011 – 2012
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| Keywords | 幹細胞 |
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| Research Abstract |
I set out to develop a novel technology that enables to derivate iPS cells which can differentiate into desired differentiated cell types without cell cloning during reprograming. I constructed gene vectors that is required for this system, and developed a novel hematopoietic cell differentiation system which can efficientlyderivate immature hematopoietic precursors and mature hematopoietic cells. I introduced constructed vectors into iPS cells and recovering clones which have modified allele in AAVS1 site by homologous recombination.
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[Journal Article] Robust and highly-efficient differentiation of functional monocytic cells from human pluripotent stem cells under serum- and feeder cell- free conditions.2013
Author(s)
Yanagimachi MD, Niwa A, Tanaka T, Ozaki F, Nishimoto S, Murata Y, Yasumi T, Ito J, Tomida S, Oshima K, Asaka I, Goto H, Heike T, Nakahata T, Saito MK*.
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Journal Title
PLoS One
Volume: 8(4)
Pages: e59243
DOI
Peer Reviewed
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[Journal Article] Induced pluripotent stem cells from CINCA syndrome patients as a model for dissecting somatic mosaicism and drug discovery.2012
Author(s)
Tanaka T, Takahashi K, Yamane M, Tomida S, Nakamura S, Oshima K, Niwa A, Nishikomori R, Kambe N, Hara H, Mitsuyama M, Morone N, Heuser JE, Yamamoto T, Watanabe A, Sato-Otsubo A, Ogawa S, Asaka I, Heike T, Yamanaka S, Nakahata T, Saito MK*.
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Journal Title
Blood.
Volume: 120(6)
Pages: 1299-308
DOI
Peer Reviewed
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[Journal Article] Frequent somatic mosaicism of NEMO in T cells of patients with X-linked anhidrotic ectodermal dysplasia with immunodeficiency.2012
Author(s)
Kawai T, Nishikomori R, Izawa K, Murata Y, Tanaka N, Sakai H, Saito M, Yasumi T, Takaoka Y, Nakahata T, Mizukami T, Nunoi H, Kiyohara Y, Yoden A, Mutara T, Sasaki S, Ito E, Akutagawa H, Kawai T, Imai C, Okada S, Kobayashi M, Heike T.
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Journal Title
Blood.
Volume: 119(23)
Pages: 5458-66
DOI
Peer Reviewed
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[Journal Article] Multiple reversions of an IL2RG mutation restore T cel function in an X -linked severe combined immunodeficiency patient.2012
Author(s)
Kawai T, Saito M, Nishikomori R, Yasumi T, Izawa Km Murakami T, Okamoto S, Mori Y, Nakagawa N, Imai K, Nonoyama S, Wada T, Ohmori K, Nakahata T, Heike T.
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Journal Title
J Clin Immunol.
Volume: 32(4)
Pages: 690-7
DOI
Peer Reviewed
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[Journal Article] Detection of Base Substitution-Type Somatic Mosaicism of the NLRP3 Gene with >99.9% Statistical Confidence by Massively Parallel Sequencing.2012
Author(s)
Izawa K, Hijikata A, Tanaka N, Kawai T, Saito MK, Goldbach-Mansky R, Aksentijevich I, Yasumi T, Nakahata T, Heike T, Nishikomori R, Ohara O.
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Journal Title
DNA Res.
Volume: 19(2)
Pages: 143-52
DOI
Peer Reviewed
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[Journal Article] Identification of hepatic niche harboring human acute lymphoblastic leukemic cells via the SDF-1/CXCR4 axis.2011
Author(s)
Kato I, Niwa A, Heike T, Fujino H, Saito MK, Umeda K, Hiramatsu H, Ito M, Morita M, Nishinaka Y, Adachi S, Ishikawa F, Nakahata T.
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Journal Title
PLoS One.
Volume: 6(11)
Pages: e27042
DOI
Peer Reviewed
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