2012 Fiscal Year Final Research Report
How do non-signalsequence small peptides transmit intercellular signals?
| Project/Area Number |
23657152
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Developmental biology
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| Research Institution | Kobe University |
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Principal Investigator |
KAGEYAMA Yuji 神戸大学, 遺伝子実験センター, 准教授 (90335480)
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| Project Period (FY) |
2011 – 2012
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| Keywords | ショウジョウバエ / 短鎖ペプチ / 細胞間シグナル伝達 / 発生遺伝 |
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| Research Abstract |
The polished ricegene in Drosophilaencodes extremely small 11 or 32-aa long peptides (PRI peptides) that do not include any signal sequences, although genetic analysis have shown that prishows non-cell autonomous effects to neighboring cells. To elucidate molecular mechanisms underlying the non-cell autonomous signaling by the prigene, we tried to identify PRI peptides-associating proteins using cultured cells. Although we failed to raise specific antibody against PRI peptides, fusion peptides with molecular tags were effectively precipitated with anti-tag antibodies. We identified several proteins that are co-precipitated with PRI fusion peptides.
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