2013 Fiscal Year Final Research Report
An approach for finding of novel biological clock focusing on the segmentation of the pharyngeal arch
| Project/Area Number |
23657153
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Developmental biology
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| Research Institution | Okazaki Research Facilities, National Institutes of Natural Sciences |
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Principal Investigator |
TAKADA Shinji 大学共同利用機関法人自然科学研究機構(岡崎共通研究施設), 岡崎統合バイオサイエンスセンター, 教授 (60206753)
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| Project Period (FY) |
2011 – 2013
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| Keywords | 形態形成 / 分節形成 / 分子時計 / 咽頭弓 |
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| Research Abstract |
The segmentation of pharyngeal arches requires Ripply, encoding an adaptor protein, which is expressed in the pharyngeal endoderm and ectoderm. Ripply3 is periodically expressed in caudal end of developing pharyngeal endoderm, where segmental morphogenesis takes place repeatedly. In this study, we identified a promoter element required for periodical expression of Ripply3 at the caudal end of pharyngeal endoderm by generating transgenic mice carrying Ripply3 promoter-EGFP fusion gene. We also showed that a feedback regulatory loop composed by Tbx1 and Ripply3 is critical for generating this periodicity by reporter analyses using culture cells and Ripply3 knock out mouse embryos. These results strongly suggest that a feedback interaction between Tbx1 and Ripply3 is important for periodical gene expression during segmentation of the pharyngeal endoderm, which has a slower periodicity than that in the segmentation of somites.
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[Journal Article] Ripply 3, a Tbx repressor, is required for development of the pharyngeal apparatus and its its derivatives in mice2011
Author(s)
Okubo, T., Kawamura, A., Takahashi, J., Yagi, H., Morishima, M., Matsuoka, R., & Takada, S
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Journal Title
Development
Volume: 138
Pages: 339-348
Peer Reviewed
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