Origin and evolution of allosteric riboswitches

2013 Fiscal Year Final Research Report

• Project/Area Number: 23657160
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Evolutionary biology
• Research Institution: Tokyo University of Science
• Principal Investigator: TAMURA Koji 東京理科大学, 基礎工学部, 教授 (30271547)
• Project Period (FY): 2011 – 2013
• Keywords: 機能進化 / RNA / リボスイッチ / アロステリック

Research Abstract

The Bacillus subtilis glycine riboswitch comprises tandem glycine-binding aptamers and regulates transcription via the sensing of glycine. And it shows allosteric effect by binding of one glycine to one aptamer. Here, I found that the riboswitch behaves in a "glycine-independent" manner in the presence of polyethylene glycol and ethylene glycol. The effect is related to the formation of a terminator stem within the expression platform under such conditions. This provided new perspective about the evolution of riboswitch and acquisition of allostericity that molecule-specific functions of riboswitches would have come later, after the formation of less specific domain in riboswitches.

Research Products

• [Journal Article] RNA evolution conjectured from tRNA and riboswitches (2014)
  • Author(s): Tamura, K
  • Journal Title: Hypothesis Volume: (in press)
  • URL: http://www.hypothesisjournal.com
  • Peer Reviewed
• [Journal Article] Glycols modulate terminator stem stability and ligand-dependency of a glycine riboswitch (2013)
  • Author(s): Hamachi, K., Hayashi, H., Shimamura, M., Yamaji, Y., Kaneko, A., Fujisawa, A., Umehara, T. and Tamura, K
  • Journal Title: BioSystems Volume: 113 Pages: 59-65
  • DOI: 10.1016/j.biosystems.2013.05.004
  • Peer Reviewed
• [Journal Article] RNA tetraplex as a primordial peptide synthesis scaffold (2012)
  • Author(s): Umehara, T., Kitagawa, T., Nakazawa, Y., Yoshino, H., Nemoto, R. and Tamura, K
  • Journal Title: BioSystems Volume: 109 Pages: 145-150
  • DOI: 10.1016/j.biosystems.2012.03.003
  • Peer Reviewed
• [Journal Article] Ribosome evolution : Emergence of peptide synthesis machinery (2011)
  • Author(s): Tamura, K
  • Journal Title: J. Biosci Volume: 36 Pages: 921-928
  • URL: http://www.ias.ac.in/jbiosci/dec2011/921.pdf
  • Peer Reviewed
• [Journal Article] Molecular basis for chiral selection in RNA aminoacylation (2011)
  • Author(s): Tamura, K
  • Journal Title: Int. J. Mol. Sci Volume: 12 Pages: 4745-4757
  • DOI: 10.3390/ijms12074745
  • Peer Reviewed
• [Presentation] tRNA とリボスイッチから見たRNA の進化 (2014)
  • Author(s): 浜地心, 楳原琢哉, 田村浩二
  • Organizer: 生命の起原および進化学会第39回学術講演会
  • Place of Presentation: 広島修道大学
  • Year and Date: 2014-03-15
• [Presentation] Domain structures and evolution of tRNA, aminoacyl tRNA synthetases, and riboswitches (2013)
  • Author(s): Kokoro Hamachi, Takuya Umehara, Koji Tamura
  • Organizer: AARS2013 2013 9th International Symposium on Aminoacyl-tRNA Synthetases
  • Place of Presentation: The Prince Hakone
  • Year and Date: 2013-10-06
• [Presentation] Evolution of riboswitches conjectured from a glycine-independent glycine riboswitch (2013)
  • Author(s): Kokoro Hamachi, Takuya Umehara, Koji Tamura
  • Organizer: 第86回日本生化学会大会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2013-09-13
• [Presentation] グリコールがグリシンリボスイッチのグリシン依存性を変調する (2013)
  • Author(s): 浜地心, 楳原琢哉, 田村浩二
  • Organizer: 東京理科大学総合研究機構・RNA 科学総合研究センター公開シンポジウム「RNA worldへの階梯」
  • Place of Presentation: 東京理科大学・葛飾キャンパス
  • Year and Date: 2013-07-17
• [Presentation] グリシンリボスイッチの発現調節部位とRNA ワールド (2013)
  • Author(s): 濱地心, 楳原琢哉, 田村浩二
  • Organizer: 生命の起原および進化学会第38回学術講演会
  • Place of Presentation: 九州大学箱崎キャンパス
  • Year and Date: 2013-03-15
• [Book] 生命分子を統合するRNAーその秘められた役割と制御機構 (2013)
  • Author(s): 田村浩二
  • Total Pages: 23-29
  • Publisher: 羊土社
• [Remarks]
  • URL: http://kojitamura.web.fc2.com/