2013 Fiscal Year Final Research Report
Critical analyses of "Sex" in homothallic Closterium peracerosum-strigosum-littorale complex
| Project/Area Number |
23657161
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Evolutionary biology
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| Research Institution | Japan Women's University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
TSUCHIKANE Yuki 日本女子大学, 理学部, 助教 (20434152)
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| Project Period (FY) |
2011 – 2013
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| Keywords | ミカヅキモ / 有性生殖 / ホモタリズム / ヘテロタリズム / 性分化 / 性決定 |
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| Research Abstract |
In Closterium peracerosum-strigosum-littorale complex, two types of conjugation produce zygotes; one is a conjugation between two complementary mating-type cells (mt+ and mt-) and the other is a conjugation between clonal cells. The former is referred to as heterothallism and the latter as homothallism.
Heterothallic mating group II-B and homothallic strains (naga37) are phylogenetically closely related. The formation of hybrid zygotes between the homothallic cells and either heterothallic cells was confirmed. These results suggest that at least some of the homothallic gametangial cells possess the same characteristics as heterothallic mt- and/ or mt+ cells. On the other hand, progenies derived from the hybrid zygotes could not be obtained.
Total RNA was collected from cells in several asexual and sexual reproductive stages and large scale RNAseq data was obtained by using new generation sequencer Hiseq2000. Finally, 132329 transcripts including many isoforms were established.
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Research Products
(26 results)
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[Journal Article] Characterization of sexual reproductive processes in Chara braunii (Charales, Charophyceae)2014
Author(s)
Sato, M., Sakayama, H., Sato, M., Ito, M., Sekimoto, H
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Journal Title
Phycol. Res
Volume: (in press)
Peer Reviewed
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[Journal Article] The evolution of Male-Female sexual dimorphism predates the gender-based divergence of mating locus gene Mat3/RB2013
Author(s)
Hiraide, R., Kawai-Toyooka, H., Hamaji, T., Matsuzaki, R., Kawafune, K., Abe, J., Sekimoto, H., Umen, J., Nozaki, H
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Journal Title
Mol. Biol. Evol
Volume: 30(5)
Pages: 1038-1040
DOI
Peer Reviewed
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[Journal Article] Stable nuclear transformation of Closterium peracerosum-strigosum-littorale complex2011
Author(s)
Abe, J., Hori, S., Tsuchikane, Y., Kitao, N., Kato, M., Sekimoto, H
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Journal Title
Plant Cell Physiol
Volume: 52(9)
Pages: 1676-1685
DOI
Peer Reviewed
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[Journal Article] Conjugation processes of Penium margaritaceum (Zygnematophyceae, Zygnematophyta)2011
Author(s)
Tsuchikane, Y., Tsuchiya, M., Kokubun, Y., Abe, J., Sekimoto, H
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Journal Title
Phycol. Res
Volume: 59
Pages: 74-82
DOI
Peer Reviewed
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[Presentation] A receptor-like kinase responsible for the progress of sexual reproduction of a charophycean alga, Closterium peracerosum-strigosum-littorale complex2013
Author(s)
Sekimoto, H., Hirano, N., Marukawa, Y., Abe, J., Tsuchikane, Y
Organizer
10th International phycological congress
Place of Presentation
Orlando, Florida USA
Year and Date
2013-08-06
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[Presentation] Intercellular communication in sexual reproduction of a unicellular charaphycean alga, Closterium peracerosum-strigosum-littorale complex2012
Author(s)
Sekimoto, H., Hirano, N., Marukawa, Y., Tsuchikane, Y., Abe, J
Organizer
International Symposium on the Mechanisms of Sexual Reproduction in Animals and Plants
Place of Presentation
Nagoya
Year and Date
2012-11-16
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