Evolution of visual opsin gene expression through gene duplication manifested in fish and primates

2012 Fiscal Year Final Research Report

• Project/Area Number: 23657164
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Physical anthropology
• Research Institution: The University of Tokyo
• Principal Investigator: KAWAMURA Shoji 東京大学, 大学院・新領域創成科学研究科, 教授 (40282727)
• Project Period (FY): 2011 – 2012
• Keywords: オプシン / 視物質 / ゼブラフィッシュ / グッピー / トランスジェニック / 発現制御領域 / 霊長類 / 国際情報交換

Research Abstract

Functional differentiation of visual opsin genes through gene duplication was investigated for fish and primates as a study model, in terms of differentiation in gene expression and absorption spectra. We contributed to understanding of retinal development by visualizing a subset of photoreceptor cells through opsin-class-specific promoters. We found that guppy LWS opsin genes have undergone genetic differentiation among wild populations. We showed that L and M opsin genes of primates have experienced homogenization between them by gene conversion in introns and hybridization, while spectral difference between L and M opsins appear to have been maintained by purifying selection.

Research Products

• [Journal Article] Characterization of transgenic zebrafish lines that express GFP in the retina, pineal gland, olfactory bulb, hatching gland, and optic tectum. (2013)
  • Author(s): Fang, W., Bonaffini, S., Zou, J., Wang, X., Zhang, C., Tsujimura, T., Kawamura, S. and Wei, X.
  • Journal Title: Gene Expression Patterns Volume: 13(5-6) Pages: 150-159
  • URL: http://dx.doi.org/10.1016/j.gep.2013.02.006
  • Peer Reviewed
• [Journal Article] Bipolar cell-photoreceptor connectivity in the zebrafish (Danio rerio) retina. (2012)
  • Author(s): Li, Y. N., Tsujimura, T., Kawamura, S. and Dowling, J. E.
  • Journal Title: The Journal of Comparative Neurology Volume: 520(16) Pages: 3786-3802
  • URL: http://dx.doi.org/10.1002/cne.23168
  • Peer Reviewed
• [Journal Article] 視覚センサー遺伝子の適応と進化多様性:魚類から霊長類まで (2012)
  • Author(s): 河村正二
  • Journal Title: 化学と生物 Volume: 50(5) Pages: 325-336
  • Peer Reviewed
• [Journal Article] Gene conversion and purifying selection shape nucleotide variation in gibbon L/M opsin genes. (2011)
  • Author(s): Hiwatashi, T., Mikami, A., Katsumura, T., Suryobroto, B., Perwitasari-Farajallah, D., Malaivijitnond, S., Siriaroonrat, B., Oota, H., Goto, S. and Kawamura, S.
  • Journal Title: BMC Evolutionary Biology Volume: 11 Pages: 312
  • URL: http://dx.doi.org/10.1186/1471-2148-11-312
  • Peer Reviewed
• [Presentation] 魚類をモデルとした色覚進化の適応的柔軟性の検討 (2013)
  • Author(s): 河村正二
  • Organizer: 三重大学COE-A(世界に誇れる世界トップレベルの研究拠点)「魚類をモデルとした生物多様性と次世代型ポストゲノム教育研究拠点」シンポジウム「色覚・光受容機能の多様性・環境応答とその応用」
  • Place of Presentation: 三重大学生物資源学部、津
  • Year and Date: 2013-01-23
• [Presentation] Molecular mechanism and adaptive significance of evolutionary diversification of visual opsins (2012)
  • Author(s): Kawamura, S.
  • Organizer: The 89th Annual Meeting of the Physiological Society of Japan: Symposium 38: Functional evolution of sensing receptors: Toward the comprehensive understanding by fusion of research in the different fields (Organized by Makoto Tominaga and Hioo Imai), Shinshu University Matsumoto Campus, Matsumoto, Japan
  • Place of Presentation: (Oral) (Invited)
  • Year and Date: 20120329-31
• [Presentation] 魚類と霊長類の研究から見えてきた色覚進化の秘密 (2012)
  • Author(s): 河村正二
  • Organizer: 日本塗装技術協会第27回塗料・塗装研究発表会、東京大学生産技術研究所、駒場リサーチキャンパス・コンベンションホール
  • Place of Presentation: 駒場
  • Year and Date: 2012-03-09
• [Presentation] Evolution of visual opsin genes in fish and primates (2011)
  • Author(s): Kawamura, S.
  • Organizer: 15th Evolutionary Biology Meeting at Marseilles (15th EBM), Regional Center of Educational Documentation ("Centre Regional de Documentation Pedagogique")
  • Place of Presentation: Marseilles, France
  • Year and Date: 20110927-30
• [Presentation] Evolutionary study of vertebrate color vision: from fish transgenesis to field primatology, and to human variation (2011)
  • Author(s): Kawamura, S.
  • Organizer: Annual Conference of Soceity for Molecular Biology and Evolution2011 (SMBE 2011)
  • Place of Presentation: Kyoto University (Clock Tower Centennial Hall and Shiran Kaikan) and Miyako Messe, Kyoto, Japan
  • Year and Date: 20110726-30
• [Presentation] 色覚進化研究の人類学的展開:魚類と霊長類に見られる視覚光センサーの進化多様性を通して (2011)
  • Author(s): 河村正二
  • Organizer: 北里大学海洋分子生物学特別セミナー
  • Place of Presentation: 北里大学相模原キャンパス、神奈川
  • Year and Date: 2011-12-02
• [Book] Polymorphism and adaptation of primate colour vision. In: Evolutionary Biology Mechanisms and Trends (Pontarotti, P. ed.) (2012)
  • Author(s): *Melin, A. D., Hiramatsu, C., Fedigan, L.M., Schaffner, C. M, Aureli, F. and*Kawamura, S.
  • Total Pages: 225-241
  • Publisher: Springer-Verlag, Berlin Heidelberg.
  • URL: http://dx.doi.org/10.1007/978-3-642-30425-5_13
• [Book] 原著:Carl Zimmer (2010). The Tangled Bank: An Introduction to Evolution, Roberts and Company Publishers, Greenwood Village, Colorado.第 8 章コラム:霊 長類型 3 色型色覚をめぐる「謎」. In:進化:生命のたどる道 (長谷川眞理子 日本語版監修) (2012)
  • Author(s): *河村正二
  • Total Pages: 208-209
  • Publisher: 岩波書店
  • URL: http://www.iwanami.co.jp/moreinfo/0054670/top.html
• [Book] Polymorphic color vision in primates: evolutionary considerations. In: Post-Genome Biology of Primates (2012)
  • Author(s): *Kawamura, S., Hiramatsu, C., Melin, A.D., Schaffner, C. M, Aureli, F. and Fedigan, L. M.
  • Total Pages: 93-120
  • Publisher: Hirai, H., Imai, H. and Go, Y. eds.
  • URL: http://dx.doi.org/10.1007/978-4-431-54011-3_7
• [Remarks]
  • URL: http://www.jinrui.ib.k.u-tokyo.ac.jp/kawamura-home.htmlhttp://www.jinrui.ib.k.u-tokyo.ac.jp/kawamura-home-E.html