Analysis of proteins specifically produced in the tissues during aerenchyma formation in maize

2012 Fiscal Year Final Research Report

• Project/Area Number: 23658008
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Breeding science
• Research Institution: Nagoya University
• Principal Investigator: NAKAZONO Mikio 名古屋大学, 生命農学研究科, 教授 (70282697)
• Project Period (FY): 2011 – 2012
• Keywords: レーザーマイクロダイセクション / タンパク質 / 通気組織

Research Abstract

Aerenchyma enables internal aeration between shoots and roots, and its formation is therefore important for the adaptation of plants to excess water environments. I examined optimal conditions for comprehensive proteome analysis using the cortex isolated by laser microdissection (LM) from maize roots. I determined an optimal fixative for fixation of tissues. Moreover, I detected Alcohol dehydrogenase protein by western blotting using LM-isolated cortex proteins. However, I failed to obtain enough amounts of proteins that are required for comprehensive proteome analysis.

Research Products

• [Journal Article] Enhanced formation of aerenchyma and induction of a barrier to radial oxygen loss in adventitious roots of Zea nicaraguensis contribute to its waterlogging tolerance as compared with maize (Zea mays ssp. mays) (2012)
  • Author(s): Abiko, T., Kotula, L., Shiono, K., Malik, A.I., Colmer, T.D. and Nakazono, M.
  • Journal Title: Plant, Cell & Environment Volume: 35 Pages: 1618-1630
  • DOI: DOI:10.1111/j.1365-3040.2012.02513.x
  • Peer Reviewed
• [Journal Article] Screening of candidate genes associated with constitutive aerenchyma formation in adventitious roots of the teosinte Zea nicaraguensis (2012)
  • Author(s): Abiko, T., Obara, M., Abe, F., Kawaguchi, K., Oyanagi, A., Yamauchi, T. and Nakazono, M
  • Journal Title: Plant Root Volume: 6 Pages: 19-27
  • DOI: DOI:10.3117/plantroot.6.19
  • Peer Reviewed
• [Journal Article] AhNRAMP1 iron transporter is involved in iron acquisition in peanut (2012)
  • Author(s): Xiong, H., Kobayashi, T., Kakei, Y., Senoura, T., Nakazono, M., Takahashi, H., Nakanishi, H., Shen, H., Duan, P., Guo, X., Nishizawa, N.K. and Zuo, Y
  • Journal Title: Journal of Experimental Botany Volume: 63 Pages: 4437-4446
  • DOI: DOI:10.1093/jxb/ers117
  • Peer Reviewed
• [Journal Article] Cell-type specific transcriptome analysis in nodules of Lotus japonicas (2012)
  • Author(s): Takanashi, K., Takahashi, H., Sakurai, N., Sugiyama, A., Shibata, D., Nakazono, M. and Yazaki, K
  • Journal Title: Molecular Plant-Microbe Interactions Volume: 25 Pages: 869-876
  • DOI: DOI:10.1094/MPMI-01-12-0011-R
  • Peer Reviewed
• [Journal Article] Acquisition of aluminum tolerance by modification of a single gene in barley (2012)
  • Author(s): Fujii, M., Yokosho, K., Yamaji, N., Saisho, D., Yamane, M., Takahashi, H., Sato, K., Nakazono, M. and Ma, J.F
  • Journal Title: Nature Communications Volume: 3 Pages: 713
  • DOI: DOI:10.1038/ncomms1726
  • Peer Reviewed
• [Journal Article] Genome-wide transcriptome dissection of the rice root system: Implications for developmental and physiological functions (2012)
  • Author(s): Takehisa, H., Sato, Y., Igarashi, M., Abiko, T., Antonio, B., Kamatsuki, K., Minami, H., Namiki, N., Inukai, Y., Nakazono, M. and Nagamura, Y
  • Journal Title: Plant Journal Volume: 69 Pages: 126-140
  • DOI: DOI:10.1111/j.1365-313X.2011.04777.x
  • Peer Reviewed
• [Journal Article] Identification of genes expressed in maize root cortical cells during lysigenous aerenchyma formation using laser microdissection and microarray analyses (2011)
  • Author(s): Rajhi, I., Yamauchi, T., Takahashi, H., Nishiuchi, S., Shiono, K., Watanabe, R., Mliki, A., Nagamura, Y., Tsutsumi, N., Nishizawa, N.K. and Nakazono, M.
  • Journal Title: New Phytologist Volume: 190 Pages: 351-368
  • DOI: DOI:10.1111/j.1469-8137.2010.03535.x
  • Peer Reviewed
• [Journal Article] Comprehensive network analysis of anther-expressed genes in rice by the combination of 33 laser microdissection and 143 spatiotemporal microarrays (2011)
  • Author(s): Aya, K., Suzuki, G., Suwabe, K., Hobo, T., Takahashi, H., Shiono, K., Yano, K., Tsutsumi, N., Nakazono, M., Nagamura, Y., Matsuoka, M. and Watanabe, M
  • Journal Title: PLOS ONE Volume: 6 Pages: e26162
  • DOI: DOI:10.1371/journal.pone.0026162
  • Peer Reviewed
• [Journal Article] Cell division and cell elongation in the coleoptile of rice alcohol dehydrogenase 1-deficient mutant are reduced under complete submergence (2011)
  • Author(s): Takahashi, H., Saika, H., Matsumura, H., Nagamura, Y., Tsutsumi, N., Nishizawa, N.K. and Nakazono, M
  • Journal Title: Annals of Botany Volume: 108 Pages: 253-261
  • DOI: DOI:10.1093/aob/mcr137
  • Peer Reviewed
• [Journal Article] Contrasting dynamics of radial O2-loss barrier induction and aerenchyma formation in rice roots of two lengths (2011)
  • Author(s): Shiono, K., Ogawa, S., Yamazaki, S., Isoda, H., Fujimura, T., Nakazono, M. and Colmer, T.D
  • Journal Title: Annals of Botany Volume: 107 Pages: 89-99
  • DOI: DOI:10.1093/aob/mcq221
  • Peer Reviewed
• [Presentation] トウモロコシにおける通気組織形成時のエチレン応答性遺伝子の発現解析 (2012)
  • Author(s): 高橋宏和、山内卓樹、Imene Rajhi、長村吉晃、堤伸浩、西澤直子、中園幹生
  • Organizer: 第122回 日本育種学会講演会
  • Place of Presentation: 京都
  • Year and Date: 20120924-25
• [Presentation] Study of aerenchyma formation in maize roots during waterlogged conditions. Tunisian Japanese Symposium on Science (2011)
  • Author(s): Imene Rajhi, Takaki Yamauchi, Hirokazu Takahashi, Shunsaku Nishiuchi, Katsuhiro Shiono, Ryosuke Watanabe, Ahmed Mliki, Yoshiaki Nagamura, Nobuhiro Tsutsumi, Naoko K. Nishizawa, Mikio Nakazono
  • Organizer: Society and Technology 11
  • Place of Presentation: Yasmine Hammamet, Tunisia.
  • Year and Date: 20111111-13
• [Presentation] トウモロコシの通気組織形成に関与するエチレン応答性遺伝子の探索 (2011)
  • Author(s): 高橋宏和、山内卓樹、Imene. Rajhi、長村吉晃、堤伸浩、西澤直子、中園幹生
  • Organizer: 第120回 日本育種学会講演会
  • Place of Presentation: 福井
  • Year and Date: 20110923-24