2012 Fiscal Year Final Research Report

Research for evaluation and usage of biological nitrogen fixation in rice

Research Project

Project/Area Number	23658017
Research Category	Grant-in-Aid for Challenging Exploratory Research
Allocation Type	Multi-year Fund
Research Field	Crop science/Weed science
Research Institution	Kyoto University
Principal Investigator	INOUE Hiromo 京都大学, 大学院・農学研究科, 講師 (40260616)
Project Period (FY)	2011 – 2012
Keywords	栽培体系 / 生物的窒素固定
Research Abstract	Rice cultivar Taichung 65, Nipponbare, Kasalath and C5444 were cultivated in the pots using masa soil. By using 15N labeled (NH4)2SO4 as nitrogen fertilizer and pure water as irrigation through total rice growth term, the amount of biological nitrogen fixation in rice could be estimated in the pot experiment. According to this method, biological nitrogen fixation in the F2 population from the cross between Taichung 65 and C5444 was estimated and it was revealed that this agronomic character may be controlled by genetic factors. Nitrogen fixation microorganisms could be easily detected using the PCR with DNA extracted from the soils, with which rice was cultivated, as the template DNA.

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