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2012 Fiscal Year Final Research Report

Development of the virus vaccine by the elucidation of the virulence of a plant virus

Research Project

  • PDF
Project/Area Number 23658042
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Plant pathology
Research InstitutionSt. Marianna University School of Medicine

Principal Investigator

MIYOSHI Hiroshi  聖マリアンナ医科大学, 医学部, 講師 (80322519)

Co-Investigator(Kenkyū-buntansha) TOMOO Koji  大阪薬科大学, 薬学部, 准教授 (70257898)
Project Period (FY) 2011 – 2012
Keywords感染 / 増殖
Research Abstract

To elucidate the mechanism of RNA depurination, and to understand how PAP recognizes and targets various RNAs, the interactions between PAP and Turnip mosaic virus genome linked protein (VPg) were investigated. VPg can function as a cap analog in cap-independent translation, and potentially target PAP to uncapped IRES-containing RNA. In this work, fluorescence spectroscopy and HPLC techniques were used to quantitatively describe PAP depurination activity and PAP-VPg interactions. PAP binds to VPg with high affinity (29.5 nM); the reaction is enthalpically driven and entropically favored. Further, VPg is a potent inhibitor of PAP depurination of RNA in wheat germ lysate, and competes with structured RNA derived from tobacco etch virus (TEV) for PAP binding. VPg may confer an evolutionary advantage by suppressing one of the plant defense mechanisms, and also suggests the possible use of this protein against the cytotoxic activity of RIPs.

  • Research Products

    (5 results)

All 2012 2011 2008 Other

All Journal Article (2 results) (of which Peer Reviewed: 2 results) Presentation (2 results) Remarks (1 results)

  • [Journal Article] Inhibition of pokeweed antiviral protein by turnip mosaic virus genome-linked protein (VPg)2012

    • Author(s)
      Domashevskiy, A V., Miyoshi, H., Goss, D J.
    • Journal Title

      J. Biol. Chem

      Volume: Vol.287 Pages: 29729-29738

    • DOI

      DOI:10.1074/jbc.M112.367581

    • Peer Reviewed
  • [Journal Article] Replacement of Arg-386 with Gly in Dynamin 1 Middle Domain Reduced GTPase Activity and Oligomer Stability in the Absence of Lipids2008

    • Author(s)
      Takahashi, K., Otomo, M., Yamaguchi, N., Nakashima, H., and Miyoshi, H
    • Journal Title

      Biosci. Biotechnol. Biochem.

      Volume: Vol.76 Pages: 2195-2200

    • DOI

      DOI:10.1271/bbb.120462

    • Peer Reviewed
  • [Presentation] カブモザイクウィルスのゲノム結合タンパク質によるアメリカヤマゴボウ抗ウイルス蛋白の抑制2012

    • Author(s)
      三好洋,ArtemV.Domashevskiy,中島秀喜,DixieJ.Goss
    • Organizer
      第85回日本生化学会大会
    • Place of Presentation
      福岡市、福岡国際会議場
    • Year and Date
      2012-12-15
  • [Presentation] 阻害剤と変異体を用いたDynaminGTPaseActivityの解析2011

    • Author(s)
      高橋清文,大友雅広,長田賢一,山口登,中島秀喜,三好洋
    • Organizer
      第34回日本分子生物学会年会
    • Place of Presentation
      横浜市、パシフィコ横浜
    • Year and Date
      2011-12-16
  • [Remarks]

    • URL

      http://www.marianna-u.ac.jp/houjin/staff/univ/bisei.html

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Published: 2014-09-25  

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