2012 Fiscal Year Final Research Report
Virus-like particles of parasitoid wasps: functional analyses via gene knockdown and the search for genetic resources
| Project/Area Number |
23658047
|
|---|
| Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Applied entomology
|
|---|
| Research Institution | Nagoya University |
|---|
Principal Investigator |
MIURA Ken 名古屋大学, 生命農学研究科, 准教授 (60219582)
|
|---|
| Project Period (FY) |
2011 – 2012
|
| Keywords | RNA 干渉 |
|---|
| Research Abstract |
To elucidate functions of factors associated with virus-like particles (VLPs), which are used for host regulation by a parasitoid wasp Meteorus pulchricornis, mRNA repertoire of pupal venom gland that produces VLPs were comprehensively obtained. By utilizing RNA interference-based approaches, a series of knockdown wasps that produce VLPs deficient in specified factors were prepared. Through the functional analyses of these altered VLPs, two novel VLP constituents that share some physicochemical properties with each other were found to suppress immune responses of host hemocytes.
|
