2013 Fiscal Year Final Research Report
Establishment of primary silkworm strain having the capability of pupal diapause
| Project/Area Number |
23658050
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Applied entomology
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| Research Institution | Yamaguchi University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
KADONO Keiko 独立行政法人農業生物資源研究所, 昆虫科学研究領域, ユニット長 (40355722)
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| Project Period (FY) |
2011 – 2013
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| Keywords | Bombyx mandarina / pupal duration / polygene / bet hedging |
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| Research Abstract |
In order to elucidate genetic mechanisms of nonuniform pupal duration of Bombyx mandarin and establish a pupal diapause strain of B. mori, selection of individuals showing longer pupal durations from the progenies of hybrids between B. mandarin and B. mori has been conducted and resulted in a hybrid strain with nonuniform pupal duration. Segregation patterns of pupal period in reciprocal F1 and BC1 between the hybrid strain and the Daizo strain of B. mori have indicated that interactions of gene(s) on sex (Z) chromosome and recessive gene(s) on autosomes are involved in the nonuniform pupal duration. In addition, PCR analysis of BC1 genome DNA using primers designed for discrimination between chromosomes derived from the hybrid strain and those from the Daizo strain suggested that pupal duration is a quantitative trait determined by the cumulative result of many minor genes (polygenes), some of which are different between males and females.
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