2013 Fiscal Year Final Research Report
Global isolation and classification of Azospirillum species by a new method
Project/Area Number |
23658080
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Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Multi-year Fund |
Research Field |
Applied microbiology
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Research Institution | Okayama University |
Principal Investigator |
TANI Akio 岡山大学, その他部局等, 助教 (00335621)
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Co-Investigator(Renkei-kenkyūsha) |
NAKAGAWA Tomoyuki 岐阜大学, 応用生物科学部, 准教授 (70318179)
MITSUI Ryoji 岡山理科大学, 理学部, 准教授 (60319936)
|
Research Collaborator |
NURETTIN Sahin ムグラ大学(トルコ共和国), 教育学部, 教授
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Project Period (FY) |
2011 – 2012
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Keywords | 国際情報交換 / Azospirllum属細菌 / 窒素固定 / 根圏 |
Research Abstract |
Azospirillum species is azotrophic bacteria symbiotic to plant roots, and capable of promoting plant growth. Isolation of this species is difficult, and their classification and relationship between their host plants is not well elucidated. This study aims to isolate the species as many as possible, to classify them using mass-spectrometry dependent method, and to give a new insight into the classification of the species by finding new species. We found that nitrogen-free medium under micro-aerobic condition is important for their isolation, and we isolated 60 isolates. They were subjected to mass spectrometry for precise classification. We also found some species capable of promoting rice growth by 20%.
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