Metabolic engineering of sesquiterpenes in Fusarium species

2013 Fiscal Year Final Research Report

• Project/Area Number: 23658084
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Applied microbiology
• Research Institution: Nagoya University
• Principal Investigator: MAKOTO Kimura 名古屋大学, 生命農学研究科, 准教授 (20261167)
• Project Period (FY): 2011 – 2013
• Keywords: 代謝改変

Outline of Final Research Achievements

To replace the trichodiene synthase gene (Tri5) in the trichothecene gene cluster of Fusarium graminearum with a codon-optimized synthetic amorpha-4,11-diene synthase gene (ADS), we established a genome engineering technique of the fungus using the positive and negative selection system with hph::tk, a fusion gene of the hygromycin phosphotransferase gene (hph) and thymidine kinase gene (tk). At the same time, we have identified compound 1 that markedly enhances sesquiterpene production by screening of compounds that elevate the level of trichothecene production. By employing the use of these tools, a transgenic F. graminearum strain carrying ADS was established, and the expression level of Tri4 and production level of the precursor amorphadiene are under investigation.

Free Research Field

農学