2012 Fiscal Year Final Research Report
Biochemical regulation of fermentation heat and its application towaste heat recovery power generation
| Project/Area Number |
23658090
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Applied biochemistry
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| Research Institution | Nagoya University |
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Principal Investigator |
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| Project Period (FY) |
2011 – 2012
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| Keywords | 藍 / 発酵熱 / Aspergillus oryzae |
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| Research Abstract |
We studied the fermentation process of indigo leaves to understand the mechanism of theproduction of fermentation heat. The indigo leave fermentation can proceed by mixingthe dry indigo leaves and water. With the laboratory scale, the temperature of thefermented leaves increased up to 42℃ under the external temperature of 30℃. Throughthe analyses of the metagenome of the fermented leaves and the cultivation of the washfluid of the indigo leaves, we found several species of bacteria and fungus such as Pantoeacalida、Pantoea dispersa、Pantoea agglomerans、Pseudomonas alcaligenes、Sanguibactermarinus、Bacillus amyloliquefaciense、Bacillus circilans、Bacillus subtilis、Aspergillusoryzae, Mucor circinnelloides, Cryptococcus vishniacii, and Lichitheimia lamosa, andisolated some of them. When the autoclaved indigo leaves were inoculated with thesemicroorganisms, the temperature of the leaves inoculated with Aspergillus oryzae reachedto that comparable to the fermented indigo leaves. Any other microorganisms gave no sucha thermal elevation. Fermentation heat production could be obtained when the indigoleaves were fermented in the presence of penicillin and streptomycin. In contrastaddition of Nystatin, an antifungal compound, inhibited the heat production. Theseresults suggest that A. oryzae contributes to the fermentation heat production in theindigo leave fermentation.
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Research Products
(1 results)
